CV
Carmen Valente
  • Institute of Protein Biochemistry (CNR), Italy
Research fields
  • Cell biology
Preparation of Golgi Membranes from Rat Liver
Authors:  Carmen Valente, Gabriele Turacchio, Stefania Spanò, Alberto Luini and Daniela Corda, date: 11/20/2014, view: 8633, Q&A: 0
This protocol details the isolation of enriched Golgi membranes from rat liver, using discontinuous density gradient centrifugation. This high-yield extraction method is useful for several applications, including immunoprecipitation of solubilised Golgi membrane proteins (preparation included) and electron microscopy. Protocol adapted from Leelavathi et al. (1970).
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