Antonio Leyva
  • Department of Plant Molecular Genetics, Centro Nacional de Biotecnología, Spain
Research fields
  • Plant science
Quantification of Infectious Sendai Virus Using Plaque Assay
Authors:  Narihito Tatsumoto, Takamasa Miyauchi, Moshe Arditi and Michifumi Yamashita, date: 11/05/2018, view: 6595, Q&A: 1
Sendai virus (SeV) is an enveloped, single-stranded RNA virus of the family Paramyxoviridae. SeV is a useful tool to study its infectious pathomechanism in immunology and the pathomechanism of a murine model of IgA nephropathy. Virus quantification is essential not only to determine the original viral titers for an appropriate application, but also to measure the viral titers in samples from the harvests from experiments. There are mainly a couple of units/titers for Sendai viral quantification: plaque-forming units (PFU) and hemagglutination (HA) titer. Of these, we here describe a protocol for Sendai virus plaque assay to provide PFU using LLC-MK2 cells (a rhesus monkey kidney cell lines) and Guinea pig red blood cells. This traditional protocol enables us to determine Sendai virus PFU in viral stock as well as samples from your experiments.
Sendai Virus Propagation Using Chicken Eggs
Authors:  Narihito Tatsumoto, Moshe Arditi and Michifumi Yamashita, date: 09/20/2018, view: 5800, Q&A: 0
Sendai virus is a member of the family Paramyxoviridae, and an enveloped virus with a negative-stranded RNA genome. Sendai virus is not pathogenic to humans, but for mice and can cause pneumonia in mice. Easy and efficient techniques for propagating Sendai virus are required for studying virus replication, virus-induced innate- and adaptive-immunity, Sendai-virus-based virotherapy and IgA nephropathy. Here, we describe a protocol for Sendai virus propagation using chicken eggs. This traditional protocol enables us to generate a large amount of virus enough for animal experiments as well as cell culture experiments in a relatively inexpensive way.
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