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Overview
Authored
(1)
Reviewed
(4)
Chang Ho Ho Lee
Dankook University
Research fields
Molecular biology
Peer-reviewed
Preprint
Production of Guide RNAs
in vitro
and
in vivo
for CRISPR Using Ribozymes and RNA Polymerase II Promoters
Authors:
Tao Zhang
,
Yangbin Gao
,
Rongchen Wang
and
Yunde Zhao
,
date:
02/20/2017,
view:
16904,
Q&A:
0
CRISPR/Cas9-mediated genome editing relies on a guide RNA (gRNA) molecule to generate sequence-specific DNA cleavage, which is a prerequisite for gene editing. Here we establish a method that enables production of gRNAs from any promoters, in any organisms, and
in vitro
(Gao and Zhao, 2014). This method also makes it feasible to conduct tissue/cell specific gene editing.
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