Preparation of Outer Membrane Vesicle from Escherichia coli Authors: Oh Youn Kim,
Bok Sil Hong,
Kyong-Su Park,
Yae Jin Yoon,
Seng Jin Choi,
Won Hee Lee,
Tae-Young Roh,
Yoon-Keun Kim and
Yong Song Gho,
date: 12/05/2013,
view: 21695,
Q&A: 3 Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date. Previous biochemical and proteomic studies have revealed that the Gram-negative bacteria-derived OMVs are composed of various components like outer membrane proteins, lipopolysaccharides, outer membrane lipids, periplasmic proteins, DNA, and RNA. Here, in this protocol, we describe the method to isolate the OMVs from the culture supernatant of Escherichia coli (E. coli).