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Protocols
Biochemistry
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Microbiology
Molecular Biology
Neuroscience
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Articles and Issues
Current Issue
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Overview
Authored
(2)
Christiano Tanese de Souza
Centre for Innovative Cancer Research, Ottawa Hospital Research Institute, Canada
Research fields
Immunology
Peer-reviewed
Preprint
Natural Killer Cell Transfer Assay
Authors:
Lee-Hwa Tai
,
Christiano Tanese de Souza
,
Andrew P. Makrigiannis
and
Rebecca Ann C. Auer
,
date:
08/20/2013,
view:
12033,
Q&A:
0
Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the adoptive transfer of purified NK cells into NK cell-deficient tumor bearing mice in order to establish the intrinsic functionality of NK cells.
More >
Ex vivo
Natural Killer Cell Cytotoxicity Assay
Authors:
Lee-Hwa Tai
,
Christiano Tanese de Souza
,
Andrew P. Makrigiannis
and
Rebecca Ann C. Auer
,
date:
08/20/2013,
view:
19567,
Q&A:
0
Natural Killer (NK) cells are cytotoxic lymphocytes that constitute a major component of the innate immune system. Immunosurveillance of the host by NK cells for malignant and virally-infected cells results in direct cytotoxicity and the production of cytokines to enhance the immune response. This protocol will describe the “gold standard” chromium release assay for measuring the target cell killing capacity of NK cells. Key features of this cytotoxicity assay are that it is performed with sorted NK cells as the effectors and any Major Histocompatibility Class I (MHC-I)-low or deficient tumor cell line can be used as the target cells.
More >
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