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Protocols
Biochemistry
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Overview
Authored
(1)
Darrell N. Kotton
Center for Regenerative Medicine, Boston University and Boston Medical Center, USA
Research fields
Stem cell
Peer-reviewed
Preprint
Detection of Hog1 Phosphorylation in
Candida albicans
in Response to an Antifungal Protein
Authors:
Brigitte ME Hayes
and
Nicole L van der Weerden
,
date:
09/20/2014,
view:
8536,
Q&A:
0
It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect activation of the
Candida albicans
(
C. albicans
) MAPK Hog1 by monitoring its phosphorylation in response to an antifungal protein.
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