Darrell N. Kotton
  • Center for Regenerative Medicine, Boston University and Boston Medical Center, USA
Research fields
  • Stem cell
Detection of Hog1 Phosphorylation in Candida albicans in Response to an Antifungal Protein
Authors:  Brigitte ME Hayes and Nicole L van der Weerden, date: 09/20/2014, view: 8536, Q&A: 0
It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect activation of the Candida albicans (C. albicans) MAPK Hog1 by monitoring its phosphorylation in response to an antifungal protein.
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