Wei Zheng
  • Keck Biotech Services, Yale University, USA
Research fields
  • Systems biology
Polyribosome Profiling
Author:  Wei Zheng, date: 10/05/2011, view: 11610, Q&A: 0
Preparation of Taq DNA Polymerase
Author:  Wei Zheng, date: 10/05/2011, view: 34346, Q&A: 1
qPCR of Yeast ChIP DNA
Author:  Wei Zheng, date: 09/20/2011, view: 17099, Q&A: 1
This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample (provided you have a few well-characterized binding sites) before submitting it for sequencing/microarray analysis. This protocol is designed for use with a Roche LightCycler.
Yeast in situ Hybridization
Author:  Wei Zheng, date: 09/20/2011, view: 11710, Q&A: 0
This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered.
Yeast Transcription Factor Chromatin Immunoprecipitation
Author:  Wei Zheng, date: 07/05/2011, view: 13336, Q&A: 0
This ChIP protocol was developed and improved over the years by various researchers in the Snyder lab, Stanford University, especially Anthony Borneman and Christopher Yellman. I have used this method to successfully map the genome-wide binding of transcription factors Ste12. The ChIPed DNA is suitable for downstream analysis using PCR, microarray or sequencing.
Illumina Sequencing Library Construction from ChIP DNA
Author:  Wei Zheng, date: 02/20/2012, view: 20352, Q&A: 0
The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive. The protocol described here can be used to construct high-quality sequencing libraries from chromatin immunoprecipitated DNA. It uses key reagents from third-party vendors and greatly reduces the cost in library construction for Illumina sequencing.
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