Germano Cecere
  • Department of Biochemistry and Molecular Biophysics, Columbia University, USA
Research fields
  • Biochemistry
RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans
Authors:  Germano Cecere and Alla Grishok, date: 12/20/2014, view: 14349, Q&A: 0
The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans) to detect interaction between the nuclear Argonaute CSR-1 (chromosome segregation and RNAi deficient) protein and its target nascent RNAs. We have used a transgenic strain expressing a recombinant long isoform of CSR-1 protein fused with N-terminal 3x FLAG epitope.
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