About FRET profile in biocondensates

Yes. Different biocondensates show distinct FRET efficiency profiles reflecting their molecular packing density and interaction strength.

High, stable FRET indicates tight, static interactions (gel-like or solid condensates), while low or fluctuating FRET suggests dynamic, liquid-like states.

Thus, FRET can classify condensates along a liquid-to-solid continuum and monitor phase transitions in real time.