Q&A Mar 18, 2026

Soil Extraction Plating for the Detection of Aspergillus terreus from Environmental SamplesAuthors and AffiliationsJan Schobert¹, Paul Illmer², Roya Vahedi-Shahandashti¹*, Cornelia Lass-Flörl¹*¹ ISHAM Working Group of Aspergillus terreus, ECMM Excellence Center of Mycology, Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Austria² Institute of Microbiology, University of Innsbruck, AustriaAbstractThis protocol describes a selective soil extraction plating method fo...

Q&A Mar 17, 2026

Isolation of Extracellular Vesicles from Trichomonas vaginalisAbstractThis protocol describes the isolation of extracellular vesicles (EVs) from Trichomonas vaginalis using differential ultracentrifugation. Parasites are first cultured in TYM medium supplemented with heat‑inactivated horse serum until reaching the logarithmic growth phase. To induce EV release under serum‑free conditions, cells are washed and incubated for 4 hours in serum‑free TYM base. The conditioned medium is then clarified ...

Q&A Mar 16, 2026

2-Deoxy-D-glucose (2-DG) Treatment of Zebrafish EmbryosActivation of the Mondo Pathway via MicroinjectionDickmeis Laboratory IBCS-BIP/KIT, Karlsruhe, GermanyTechniqueZebrafish Embryo MicroinjectionApplicationActivation of MondoA/MLX pathway via 2-deoxy-D-glucoseModel OrganismDanio rerio (zebrafish)Document Version1.0Last Updated13/03/2026 1. Introduction and PrincipleThis protocol describes the use of the glucose analog 2-deoxy-D-glucose (2-DG) to selectively activate the Mondo pathway in zebraf...

Q&A Mar 6, 2026

 ATAC_qPCR Dissect embryos in Ringers solution. Single neural folds (˜3000 cells) contains enough cells for testing a few primer pairs.Transfer dissected samples to a microcentifuge tube (low binding).Rinse with 300uL Accumax to remove excess salt from RingersAdd 300uL of Accumax and incubate the tissue at RT for 20-30’, rocking at orbital shaker. During this period, gently pipette the tissue up and down to help tissue start dissociate.Centrifuge 3 min 600rcf at room temperature and withdraw liq...

Q&A Mar 2, 2026

Section I. Hazardous Reagent Safety & Preparation of key solutions (section is for both protocols) Mandatory Safety Rules (read primary MSDS and review EH&S guidelines before proceeding)Fume Hood Requirement: All weighing and mixing of dry powders (PFA and NaN3) must be performed inside a certified chemical fume hood.Respiratory Protection: Per SDS guidelines, wear a well-fitted N95 mask when weighing powders to prevent inhalation of toxic dust (NaN3 can be fatal if inhaled; PFA is a car...

Q&A Feb 25, 2026

BrU-labeling and immunoprecipitation of newly synthesized RNA Anne Kruse Hollensen1, Christian Kroun Damgaard11Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark ProtocolSeeding of cellsSeed cells in eight p6 dishes per series of samples6 ml cell culture medium/dish PulseAspirate 4 ml of medium from each plate and pool medium from each series of samples. For each series of samples:For one dish (–BrU): Aspirate the remaining medium from the cells and add 3 ml of the ...

Q&A Feb 24, 2026

Hierarchical Cell Type Annotation Based on scANVIOverviewTo provide a standardized method for annotating cell types at different resolutions, this protocol describes a hierarchical cell type annotation workflow based on scANVI. The method enables multi-resolution annotation of single-cell RNA-seq datasets by:First identifying broad cell classes (primary cell types).Then refining annotations to more specific cell types within each class.Optionally extending to additional hierarchical levels.Integ...

Q&A Feb 24, 2026

Electroconvulsive Stimulation (ECS) ProcedureEri Segi-Nishida1, Katsunori Kobayashi21Department of Biological Science and Technology, Faculty of Advanced Engineering, Tokyo University of Science, Tokyo, Japan.2Department of System Neurophysiology, Wakayama Medical University, Wakayama, Japan. AbstractElectroconvulsive therapy (ECT) is an effective treatment for medication-resistant depression. Although ECT has been used for many decades, its cellular mechanism of action is not fully understood. ...

Q&A Feb 18, 2026

Group-based Minimum Spanning Tree Correlation NetworkAuthors: Camilo Espinosa Bernal, Nima Aghaeepour The R script outlined below and attached as a supplementary txt file represent a user-friendly template to generate a group-based MST of a correlation network of clinical features for which predictive multiomic models have been built. # "# Generic Multi-Omic MST (Minimum Spanning Tree) Template# ========================================================# INPUTS REQUIRED# ---------------# 1. cl...

Q&A Feb 10, 2026

Anti-acetylated tubulin IF – Nerve IFKaylee Wells April 2020  Day 1 Primary Antibody Solution:Anti-acetylated tubulin, T7451 mouse monoclonal (Located in small -20 ℃)1:1500 dilution in 0.5% BSA (BSA made in PBST) PBS x2 (10 min)Pap pen around the sections and let dry ( >1 hour)PBS (10 min)0.5% Trition X in PBST (5 min)Block in 0.5% BSA in PBST (>2 hours)Incubate with primary antibody (overnight) at 4 ℃ in humidification chamber Day 2 Secondary Antibody Solution:Goat-anti-mouse 488A11001 – ...