Direct co-culture of parasites

Staining with CellTracker Red CMTPX Dye

-Prepare the working solution starting from the 1 mM stock. Dilute 1 µL of the stock solution in 1 mL of serum-free culture medium to obtain a final concentration of 1 µM (adjust volumes according to the number of cells to be stained).
-Resuspend the cell pellet in the required volume of the 1 µM dye-containing medium. Incubate the cells for 20 minutes at 37 °C, protected from light.
-Wash the cells three times with PBS, centrifuging between each wash. For Trichomonas vaginalis, centrifuge at 3000 rpm for 10 minutes.
-After the final wash, resuspend the cells in the desired medium. Cells are ready for downstream applications.