Step 1
100 ng/10 uL DNA added 10 μL Global DNA Digestion Solution (Recipes 2) in 1.5 mL sterilized ep tube. Vortex for at least 10 s.
Step 2
Incubate the mixture at 37 ℃ for 6 h.
Step 3
The injection volume is 2 μL.
Recipe 1. 20 mM Tris-HCl buffer
20 mM Tris-HCl buffer (pH = 7.9)
100 mM NaCl
20 mM MgCl2
Recipe 2. Global DNA Digestion Solution (5 mL for 500 times)
250 U benzonase (Novogen)
300 mU phosphodiesterase I (上海生工,Sangon Biotech Co., Ltd)
200 U alkaline phosphatase (上海生工,Sangon Biotech Co., Ltd)
Added in 5 mL 20 mM Tris-HCl buffer