Ana Flores-Mireles Department of Biological Sciences, University of Notre Dame, USA, USA,
1 protocol

José Lemos Department of Oral Biology, University of Florida College of Dentistry, USA, USA,
3 protocols
Alba Blesa Universidad Francisco de Vitoria
23 protocols

Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
51 protocols

David Cisneros Umeå University
33 protocols

Sanjeeva Metikala Cincinnati Children's Hospital Medical Center
2 protocols

Reviewer
Cristina Colomer-Winter
  • Sterility Assurance Scientist, Boehringer Ingelheim
Research focus
  • Microbiology
  • Bacteriology, Biometals, Stress Tolerance, Pathogenesis, Physiology, Virulence, Pathophysiology
  • 1 Author merit

Education

PhD, University of Florida, 2018

Lab information

Disinfectant policy and validation, regulatory policy on sterilization processes, contamination control strategies, aseptic process simulation

Publications

Colomer-Winter C, Flores-Mireles AL, Kundra S, Hultgren SJ, Lemos JA. "(p)ppGpp and CodY promote Enterococcus faecalis virulence in a murine model of catheter-associated urinary tract infection." mSphere 2019 4(4).pii:e00392-19.

Colomer-Winter C, Lemos JA, Flores-Mireles AL. “Biofilm assays on fibrinogen-coated silicone catheters and 96-well polystyrene plates.” Bio Protoc. 2019 Mar;9(6):e3196.

Colomer-Winter C, Gaca AO, Chuang-Smith ON, Lemos JA, Frank KL. “Cellular levels of (p)ppGpp differentially affect the pathogenesis of infective endocarditis in Enterococcus faecalis.” Microbiology 2018 Oct;164(10):1254-1265.

Colomer-Winter C, Flores-Mireles AL, Baker SP, Frank KL, Lynch AJL, Hultgren SJ, Kitten T, Lemos JA. “Manganese acquisition is essential for virulence of Enterococcus faecalis.” PLoS Pathog. 2018 Sep;14(9):e1007102.

Colomer-Winter C, Gaca AO, Lemos JA. “Association of metal homeostasis and (p)ppGpp regulation in the pathophysiology of Enterococcus faecalis.” Infect. Immun. 2017 Jul;85(7):e00260-17.

Gaca AO, Kudrin P, Colomer-Winter C, Beljantseva J, Liu K, Anderson B, Wang JD, Rejman D, Potrykus K, Cashel M, Hauryliuk V, Lemos JA. “From (p)ppGpp to (pp)pGpp: Characterization of regulatory effects of pGpp synthesized by the Small Alarmone Synthetase of Enterococcus faecalis.” J. Bacteriol. 2015

Gaca AO, Colomer-Winter C, Lemos JA. “Many means to a common end: the intricacies of (p)ppGpp metabolism and its control of bacterial homeostasis.” Mini-review. J. Bacteriol. 2015.

Frank KL, Colomer-Winter C, Grindle SM, Lemos JA, Schlievert PM, Dunny GM. “Transcriptome analysis of Enterococcus faecalis during mammalian infection shows cells undergo adaptation and exist in a Stringent Response state.” PLoS One. 2014 Dec 29;9(12):e115839.
1 Protocol published
Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
Authors:  Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles, date: 03/20/2019, view: 3620, Q&A: 0
Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize ...
4 Protocols reviewed
Assessment of Diadenylate Cyclase and c-di-AMP-phosphodiesterase Activities Using Thin-layer and Ion Exchange Chromatography
Authors:  Andreas Latoscha, David Jan Drexler, Gregor Witte and Natalia Tschowri, date: 01/05/2021, view: 522, Q&A: 0

All living cells use cyclic nucleotides as second messengers for signal sensing and transduction. Cyclic di-3′,5′-adenosine monophosphate (c-di-AMP) is primarily involved in the control of bacterial and euryarcheal osmoadaptation and is

...
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Radioactive Assay of in vitro Glutamylation Activity of the Legionella pneumophila Effector Protein SidJ
Authors:  Alan G. Sulpizio, Jung-Ho Shin, Marena E. Minelli and Yuxin Mao, date: 10/05/2020, view: 814, Q&A: 0
The Legionella effector protein SidJ has recently been identified to perform polyglutamylation on another Legionella effector, SdeA, ablating SdeA’s activity. SidJ is a kinase-like protein that requires the small eukaryotic protein ...
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