Ralph Thomas Boettcher

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Akira Karasawa Molecular Medicine, Cornell University, USA
2 protocols

Alexandros Kokotos Weill Cornell Medicine
8 protocols

Alka Mehra Institute of Genomics and Integrative Biology
27 protocols

Aruna Bitra La Jolla institute for allergy and immunology
2 protocols

Editor

Ralph Thomas Boettcher

Project group leader, Max Planck Institute for Biochemistry

Research focus

Cell biology

1 Author merit
Reviewer merit
Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published

β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)

Author: Ralph T. Böttcher, date: 11/05/2013, view: 7855, Q&A: 0

Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...

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4 Protocols reviewed

Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites

Authors: Christina Wefers, Ghaith Bakdash, Meritxell Moreno Martin, Tjitske Duiveman-de Boer, Ruurd Torensma, Leon F.A.G. Massuger and I. Jolanda M. de Vries, date: 04/05/2017, view: 6159, Q&A: 0

Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...

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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells

Authors: Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 4528, Q&A: 0

Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...

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37 Protocols edited

Mapping the Mechanome–A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression

Authors: Vina D. L. Putra, Iman Jalilian, Madeline Campbell, Kate Poole, Renee Whan, Florence Tomasetig and Melissa L. Knothe Tate, date: 12/05/2019, view: 87, Q&A: 0

Mechanomics, the mechanics equivalent of genomics, is a burgeoning field studying mechanical modulation of stem cell behavior and lineage commitment. Analogous to mechanical testing of a living material as it adapts and evolves, mapping of the ...

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Phospho-protein Analysis in Adherent Cells Using Flow Cytometry

Authors: Renu Sharma, Amit Sharma, Atul Kumar and Bithiah Grace Jaganathan, date: 10/20/2019, view: 708, Q&A: 0

Protein phosphorylation is one of the most important post-translational modifications, which acts as a reversible on or off switch for the activity of a large number of proteins. Analyzing the phosphorylation status of different proteins can reveal ...

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