Ralph Thomas Boettcher

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Akira Karasawa Molecular Medicine, Cornell University, USA
2 protocols

Alexandros Kokotos Weill Cornell Medicine
8 protocols

Alka Mehra Institute of Genomics and Integrative Biology
24 protocols

Aruna Bitra La Jolla institute for allergy and immunology
2 protocols

Editor

Ralph Thomas Boettcher

Project group leader, Max Planck Institute for Biochemistry

Research focus

Cell biology

1 Author merit
Reviewer merit
Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published

β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)

Author: Ralph T. Böttcher, date: 11/05/2013, view: 7652, Q&A: 0

Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...

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4 Protocols reviewed

Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites

Authors: Christina Wefers, Ghaith Bakdash, Meritxell Moreno Martin, Tjitske Duiveman-de Boer, Ruurd Torensma, Leon F.A.G. Massuger and I. Jolanda M. de Vries, date: 04/05/2017, view: 5875, Q&A: 0

Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...

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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells

Authors: Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 4381, Q&A: 0

Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...

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35 Protocols edited

Subcellular Fractionation of Hela Cells for Lysosome Enrichment Using a Continuous Percoll-density Gradient

Authors: Julian M. Carosi, Kathryn J. Hattersley, Yi Cui, Zhe Yang, Rohan D. Teasdale and Timothy J. Sargeant, date: 09/20/2019, view: 330, Q&A: 0

The enrichment of lysosomes is a useful way to study their structure and function. These dynamic vesicles can be enriched from cell cultures in a variety of ways including immunoprecipitation and fluorescence-activated organelle sorting. These ...

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Tandem Affinity Purification of SBP-CBP-tagged Type Three Secretion System Effectors

Authors: Laura Berneking, Marie Schnapp, Theresa Nauth and Moritz Hentschke, date: 06/20/2019, view: 706, Q&A: 0

Identification of protein-protein interactions of bacterial effectors and cellular targets during infection is at the core to understand how bacteria manipulate the infected host to overcome the immune response. Potential interacting proteins might ...

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