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About Us
For Authors
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Submit Manuscript
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Editorial Criteria
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A peer-reviewed protocol journal. No publication fee; no access fee.
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Overview
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Reviewed
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Akira Karasawa
New York Structural Biology Center
7 protocols
Alba Blesa
Universidad Francisco de Vitoria
24 protocols
Alexandros Kokotos
Weill Cornell Medicine
16 protocols
Alka Mehra
Institute of Genomics and Integrative Biology
39 protocols
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Editor
Ralph Thomas Boettcher
Project group leader, Max Planck Institute for Biochemistry
Research focus
Cell biology
Contributions
1
Author merit
Reviewer merit
Editor merit
Publications
https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt
1 Protocol published
β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)
Author:
Ralph T. Böttcher
,
date:
11/05/2013,
view:
9256,
Q&A:
0
Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...
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5 Protocols reviewed
Correction: Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging
Author:
Andrew D. Doyle
,
date:
05/05/2020,
view:
533,
Q&A:
0
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Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites
Authors:
Christina Wefers
,
Ghaith Bakdash
,
Meritxell Moreno Martin
,
Tjitske Duiveman-de Boer
,
Ruurd Torensma
,
Leon F.A.G. Massuger
and
I. Jolanda M. de Vries
,
date:
04/05/2017,
view:
8113,
Q&A:
0
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...
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More protocols >
42 Protocols edited
Isolation and
ex vivo
Expansion of Human Limbal Epithelial Progenitor Cells
Authors:
Naresh Polisetti
,
Günther Schlunck
,
Thomas Reinhard
,
Friedrich E. Kruse
and
Ursula Schlötzer-Schrehardt
,
date:
09/20/2020,
view:
875,
Q&A:
0
Limbal stem cell transplantation has been used successfully to treat patients with limbal stem cell deficiency all over the world. However, long term clinical results often proved less satisfactory due to the low quality of the graft or inadequate ...
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Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the
trans
Golgi Network
Authors:
Xiao Tang
,
Feng Yang
and
Yusong Guo
,
date:
03/05/2020,
view:
1807,
Q&A:
0
Protein sorting at the
trans
Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins ...
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