Ralph Thomas Boettcher

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Akira Karasawa Molecular Medicine, Cornell University, USA
2 protocols

Alexandros Kokotos Weill Cornell Medicine
4 protocols

Alka Mehra Institute of Genomics and Integrative Biology
21 protocols

Chris Tibbitt Karolinska Institutet
3 protocols

Editor

Ralph Thomas Boettcher

Project group leader, Max Planck Institute for Biochemistry

Research focus

Cell biology

1 Author merit
Reviewer merit
Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published

β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)

Author: Ralph T. Böttcher, date: 11/05/2013, view: 6620, Q&A: 0

Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...

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4 Protocols reviewed

Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites

Authors: Christina Wefers, Ghaith Bakdash, Meritxell Moreno Martin, Tjitske Duiveman-de Boer, Ruurd Torensma, Leon F.A.G. Massuger and I. Jolanda M. de Vries, date: 04/05/2017, view: 4489, Q&A: 0

Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...

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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells

Authors: Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 3456, Q&A: 0

Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...

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32 Protocols edited

Measurement of Redox States of the β3 Integrin Disulfide Bonds by Mass Spectrometry

Author: Joyce Chiu, date: 02/05/2019, view: 190, Q&A: 0

Functional disulfide bonds mediate a change in protein function in which they reside when cleaved or formed. To elucidate how a functional disulfide bond controls protein activity, it is critical that the redox state of the bond in the population of ...

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Flow Cytometry Assay for Recycling of LFA-1 in T-lymphocytes

Authors: Katarzyna Potrzebowska, Janne Lehtonen, Malin Samuelsson and Lena Svensson, date: 12/05/2018, view: 488, Q&A: 0

To enable cells to move forward, cell surface integrins are internalized into an endosomal compartment and subsequently intracellularly transported to be re-exposed at a new site on the cell membrane. Leukocytes are the fastest migrating cell type ...

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