Ralph Thomas Boettcher

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Akira Karasawa Molecular Medicine, Cornell University, USA
2 protocols

Alexandros Kokotos Weill Cornell Medicine
6 protocols

Alka Mehra Institute of Genomics and Integrative Biology
21 protocols

Aruna Bitra La Jolla institute for allergy and immunology
1 protocol

Editor

Ralph Thomas Boettcher

Project group leader, Max Planck Institute for Biochemistry

Research focus

Cell biology

1 Author merit
Reviewer merit
Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=b%C3%B6ttcher+rt

1 Protocol published

β1 Integrin Cell-surface Immunoprecipitation (Selective Immunoprecipitation)

Author: Ralph T. Böttcher, date: 11/05/2013, view: 7046, Q&A: 0

Immunoprecipitation (IP) is a widely used method to isolate a specific protein from a mixed protein sample using an antibody that exclusively binds to that particular protein. This technique allows studying protein-protein and protein-nucleic acid ...

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4 Protocols reviewed

Isolation of Mononuclear Cell Populations from Ovarian Carcinoma Ascites

Authors: Christina Wefers, Ghaith Bakdash, Meritxell Moreno Martin, Tjitske Duiveman-de Boer, Ruurd Torensma, Leon F.A.G. Massuger and I. Jolanda M. de Vries, date: 04/05/2017, view: 5017, Q&A: 0

Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer ...

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VLA-4 Affinity Assay for Murine Bone Marrow-derived Hematopoietic Stem Cells

Authors: Francesca Avemaria, Shiri Gur-Cohen, Seymen Avci and Tsvee Lapidot, date: 02/20/2017, view: 3807, Q&A: 0

Hematopoietic stem cells (HSCs) are defined by their functional ability to self-renew and to differentiate into all blood cell lineages. The majority of HSC reside in specific anatomical locations in the bone marrow (BM) microenvironment, in a ...

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33 Protocols edited

Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow

Authors: Alexander Dupuy, Lining Arnold Ju and Freda H Passam, date: 03/20/2019, view: 714, Q&A: 0

Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. Furthermore, microfluidic chips offer the benefits of ...

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Measurement of Redox States of the β3 Integrin Disulfide Bonds by Mass Spectrometry

Author: Joyce Chiu, date: 02/05/2019, view: 636, Q&A: 0

Functional disulfide bonds mediate a change in protein function in which they reside when cleaved or formed. To elucidate how a functional disulfide bond controls protein activity, it is critical that the redox state of the bond in the population of ...

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