Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

D’Acunzo, P and Levy, E(2022). Isolation of EVs from brain parenchyma. Bio-protocol Preprint. bio-protocol.org/prep1508.
D’Acunzo, P., Pérez-González, R., Kim, Y., Hargash, T., Miller, C., Alldred, M. J., Erdjument-Bromage, H., Penikalapati, S. C., Pawlik, M., Saito, M., Saito, M., Ginsberg, S. D., Neubert, T. A., Goulbourne, C. N. and Levy, E.(2021). Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome . Science Advances 7(7). DOI: 10.1126/sciadv.abe5085