Search
Editors' Choice
Editors' Choice corresponds to recently published bio-protocols (past one year) that chosen by Bio-protocol Associate Editors based on the protocols' quality, novelty, or potential impact.
Fields
  • Biochemistry
  • Cancer Biology
  • Cell Biology
  • Developmental Biology
  • Immunology
  • Microbiology
  • Molecular Biology
  • Neuroscience
  • Plant Science
  • Stem Cell
  • Systems Biology
Organisms
  • Arthropods
  • Fish
  • Fungi
  • Mammalia
  • Other Vertebrates
  • Plants
  • Prokaryotes
  • Protozoans
  • Viruses
  • Worms
Infection Process Observation of Magnaporthe oryzae on Barley Leaves
Author:  Xiao-Lin Chen, date: 05/05/2018, view: 861, Q&A: 0
Rice blast and wheat blast caused by Magnaporthe oryzae is a serious threat to rice and wheat production. Appropriate methods for observing M. oryzae infection process are important for study of the fungal infection mechanisms, plant resistance reactions, and host-M. oryzae interactions. The rice leaf sheath is commonly ...
Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels
Authors:  Seung-Kwon Ha, Govind Nair, Martina Absinta, Nicholas J. Luciano and Daniel S. Reich, date: 04/20/2018, view: 1007, Q&A: 0
In this protocol, we describe a method to visualize and map dural lymphatic vessels in-vivo using magnetic resonance imaging (MRI) and ex-vivo using histopathological techniques. While MRI protocols for routine imaging of meningeal lymphatics include contrast-enhanced T2-FLAIR and T1-weighted black-blood imaging, a more specific ...
Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
Authors:  Kuancheng Liu and Jianming Hu, date: 04/20/2018, view: 803, Q&A: 0
The hepatitis B virus (HBV) is an important global human pathogen and represents a major cause of hepatitis, liver cirrhosis and liver cancer. The HBV capsid is composed of multiple copies of a single viral protein, the capsid or core protein (HBc), plays multiple roles in the viral life cycle, and has emerged recently as a major target for ...
Imaging Cytokine Concentration Fields Using PlaneView Imaging Devices
Authors:  Alon Oyler-Yaniv and Oleg Krichevsky, date: 04/05/2018, view: 895, Q&A: 0
We describe here a method to visualize concentration fields of cytokines around cytokine-secreting cells. The main challenge is that physiological cytokine concentrations can be very low, in the pico-molar range. Since it is currently impossible to measure such concentrations directly, we rely on cell’s response to the cytokines–the ...
Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP
Authors:  Tomoharu Kanie and Peter K. Jackson, date: 04/05/2018, view: 1290, Q&A: 0
GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs). Monitoring the nucleotide exchange in vitro, together with reconstitution of direct ...
Measuring Spatiotemporal Dynamics of Odor Gradient for Small Animals by Gas Chromatography
Authors:  Akiko Yamazoe-Umemoto, Yuishi Iwasaki and Koutarou D. Kimura, date: 04/05/2018, view: 1068, Q&A: 0
Odor is the most fundamental chemical stimulus that delivers information regarding food, mating partners, enemies, and danger in the surrounding environment. Research on odor response in animals is widespread, although studies on experimental systems in which the gradient of odor concentration is quantitatively measured has been quite limited. ...
A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells
Authors:  Stephen K Jones Jr, Eric C Spivey, James R Rybarski and Ilya J Finkelstein, date: 04/05/2018, view: 930, Q&A: 0
Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection is laborious, low-throughput, and ...
Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice
Authors:  Carmelo Bellardita, Maite Marcantoni, Peter Löw and Ole Kiehn, date: 04/05/2018, view: 865, Q&A: 0
Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. ...
Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts
Authors:  Andrew M. Tidball, Preethi Swaminathan, Louis T. Dang and Jack M. Parent, date: 04/05/2018, view: 1577, Q&A: 0
For both disease and basic science research, loss-of-function (LOF) mutations are vitally important. Herein, we provide a simple stream-lined protocol for generating LOF iPSC lines that circumvents the technical challenges of traditional gene-editing and cloning of established iPSC lines by combining the introduction of the CRISPR vector ...
Organotypic Explants of the Embryonic Rodent Hippocampus: An Accessible System for Transgenesis
Authors:  Archana Iyer , Lakshmi Subramanian and Shubha Tole, date: 03/20/2018, view: 926, Q&A: 0
This protocol describes the technique of ex-vivo electroporation to target embryonic hippocampal progenitors in an organotypic slice preparation. This technique allows gene perturbation for examining developmental processes in the embryonic hippocampus while retaining the environment and connectivity of the cells. Gene perturbation can ...