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Editors' Choice
Editors' Choice corresponds to recently published bio-protocols (past one year) that chosen by Bio-protocol Associate Editors based on the protocols' quality, novelty, or potential impact.
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Peptide Feeding and Mechanical Wounding for Tomato Seedlings
Authors:  Lei Wang, Yan Wang and Georg Felix, date: 03/20/2019, view: 4879, Q&A: 0
Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host plants, it is important to have reproducible bioassays. Several assays, such as treatment of peptide ...
Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
Authors:  Kinjal Majumder, Maria Boftsi and David J Pintel, date: 03/20/2019, view: 4016, Q&A: 0
The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding principles of the cellular genome. This has led to a better understanding of the role played by ...
Straight Channel Microfluidic Chips for the Study of Platelet Adhesion under Flow
Authors:  Alexander Dupuy, Lining Arnold Ju and Freda H Passam, date: 03/20/2019, view: 12129, Q&A: 0
Microfluidic devices have become an integral method of cardiovascular research as they enable the study of shear force in biological processes, such as platelet function and thrombus formation. Furthermore, microfluidic chips offer the benefits of ex vivo testing of platelet adhesion using small amounts of blood or purified platelets. ...
Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
Authors:  Rachna Narayanan and Andrew C. Oates, date: 03/20/2019, view: 12944, Q&A: 0
In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...
Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons
Authors:  Scott Bell, Nuwan C. Hettige, Heika Silveira, Huashan Peng, Hanrong Wu, Malvin Jefri, Lilit Antonyan, Ying Zhang, Xin Zhang and Carl Ernst, date: 03/05/2019, view: 14389, Q&A: 0
Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...
Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
Authors:  Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk, date: 02/20/2019, view: 7890, Q&A: 0
For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell source for neural cell replacement but a useful model for developmental biology research. Despite ...
Imaging Higher-order Chromatin Structures in Single Cells Using Stochastic Optical Reconstruction Microscopy
Authors:  Jianquan Xu and Yang Liu, date: 02/05/2019, view: 6398, Q&A: 0
Higher-order chromatin organization shaped by epigenetic modifications influence the chromatin environment and subsequently regulate gene expression. Direct visualization of the higher-order chromatin structure at their epigenomic states is of great importance for understanding chromatin compaction and its subsequent effect on gene expression and ...
In situ, Cell-free Protein Expression on Microarrays and Their Use for the Detection of Immune Responses
Authors:  Katrin Hufnagel, Dennis Reininger, Siu Wang Ng, Nadine Gassert, Juliane K. Rohland, Soroosh Shahryarhesami, Andrea S. Bauer, Tim Waterboer and Jörg D. Hoheisel, date: 02/05/2019, view: 5048, Q&A: 0
Until recently, whole-proteome microarrays for comprehensive studies of protein interactions were mostly produced by individual cloning and cellular expression of very many open reading frames, followed by protein isolation and purification as well as array production. To overcome this cumbersome process, we have developed a method to generate ...
Measuring Homologous Recombination Rates between Chromosomal Locations in Salmonella
Authors:  Gerrit Brandis, Sha Cao and Diarmaid Hughes, date: 02/05/2019, view: 6572, Q&A: 0
Homologous recombination between two similar DNA molecules, plays an important role in the repair of double-stranded DNA breaks. Recombination can occur between two sister chromosomes, or between two locations of similar sequence identity within the same chromosome. The assay described here is designed to measure the rate of homologous ...
CRISPR-Cas9 Mediated Genome Editing in Drosophila
Authors:  Ping Peng, Xia Wang, Da Shen, Jin Sun, Yu Jia, Rong-Gang Xu, Li-Fei Zhu and Jian-Quan Ni , date: 01/20/2019, view: 9289, Q&A: 0
In recent years, great progress has been made in the research of genome editing systems, one of which is the CRISPR-Cas9 system, a powerful technology that is applied to edit animal genome. Here, we describe a CRISPR-Cas9 mediated mutation protocol for efficiently and specifically editing genes in Drosophila. In this optimized system, the ...



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