Abstract
Studying cell physiology is really important to understand their function and to determine action mechanisms taking place in these cells. Using brain slices can be sometime difficult to directly access to cells like neurons. Even if it more steps are needed to get cultured cells, this type of preparation allow a better access to neurons and provide a good way to study their basal properties. Here we describe a protocol of a short term primary neurons culture from MnPO, allowing to kept neurons in good condition to perform electrophysiological recordings.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from: Tremblay et al. (2011). This work was supported by Canadian Institutes of Health Research Grant MOP-178002.
References
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