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Feb 2020

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Tracing Nitrogen Metabolism in Mouse Tissues with Gas Chromatography-Mass Spectrometry    

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Abstract

Nitrogen-containing metabolites including ammonia, amino acids, and nucleotides, are essential for cell metabolism, growth, and neural transmission. Nitrogen metabolism is tightly coordinated with carbon metabolism in the breakdown and biosynthesis of amino acids and nucleotides. Both nuclear magnetic resonance spectroscopy and mass spectrometry including gas chromatography-mass spectrometry (GC MS) and liquid chromatography (LC MS) have been used to measure nitrogen metabolism. Here we describe a protocol to trace nitrogen metabolism in multiple mouse tissues using 15N-ammonia coupled with GC MS. This protocol includes detailed procedures in tracer injection, tissue preparation, metabolite extraction, GC MS analysis and natural abundance corrections. This protocol will provide a useful tool to study tissue-specific nitrogen in metabolically active tissues such as the retina, brain, liver, and tumor.

Keywords: GC MS, Mass spectrometry, Nitrogen metabolism, 15N tracing, Ammonia metabolism, Amino acids, Ammonia, Stable isotope tracer

Background

Nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry including mass spectrometry (GC MS) and liquid chromatography (LC MS) have been successfully used for system-wide metabolite measurements in various organisms (Fiehn, 2002 and 2016; Chokkathukalam et al., 2014). However, each method has its limitations depending on the type of studies, which include absolute quantification, metabolite properties, sensitivity, robustness, isotope analysis and cost-effectiveness (Chokkathukalam et al., 2014). Compared to NMR, MS-based approaches are more commonly used as a result of their higher sensitivity and metabolite coverage. Stable isotope labeling coupled with LC MS or GC MS allows for sensitively quantifying dynamic metabolic changes in healthy and diseased tissue or cells (Jang et al., 2018). LC MS coupled with stable nitrogen isotope reveals how glutamine nitrogen metabolism coordinates with carbon metabolism in cancer cells (Wang et al., 2019). GC MS has superior chromatographic resolution and cost-effectiveness (Jang et al., 2018). Here we have developed a robust method to quantify nitrogen-derived metabolites using stable nitrogen isotope coupled with GC MS. We identified several key metabolic features in the retina with this method, including the metabolic communications within the neural retina and between the retina and retinal pigment epithelium (RPE) (Albertino et al., 1989; Du et al., 2015 and 2016; Grenell et al., 2019; Yam et al., 2019). Recently, we used 15N-ammonia to trace nitrogen metabolism in vivo in mice and revealed tissue-specific metabolic pathways (Xu et al., 2020). In this protocol, we described procedures in nitrogen tracer injection in mice, tissue preparation, metabolite extraction, GC MS sample preparation, instrument analysis, and natural abundance corrections.


Materials and Reagents

  1. 2 ml microtube

  2. 1.5 ml tube

  3. Syringe with gauge 26 needle (BD, catalog number: 9304308)

  4. Syringe for 0.2 μm syringe filter (BD, catalog number: 7212651)

  5. ALS Syringe for GC injection (Agilent Technologies, catalog number: 5181-3354)

  6. Microtubes (Axygen Scientific, catalog number: 02820037 for 1.5 ml and catalog number: 12018028 for 2 ml)

  7. 0.2 μm syringe filter (Thermo Scientific, catalog number: 00293112)

  8. Cap for GC MS vial (Agilent Technologies, catalog number: 5182-0717)

  9. Glass inserts (Agilent Technologies, catalog number: 5181-2085, specification: 250 μl)

  10. Disposable pestle (Argos Technologies, catalog number: 7339-901)

  11. C57 B6/J mouse (Jackson Lab, catalog number: 664)

  12. Ethanol (Fisher Chemical, catalog number: 172382, LOT-specific concentration: 96%)

  13. Liquid nitrogen

  14. Methanol (Fisher Chemical, OptimaTM LC/MS Grade, catalog number: 164905)

  15. HPLC Water (Fisher Chemical, OptimaTM LC/MS Grade, catalog number:7732-18-5)

  16. Hexane (Sigma-Aldrich, HPLC Grade, catalog number: MKCF5755)

  17. Methylene Chloride (Fisher Chemical, catalog number: 163938)

  18. Helium Gas (MATHESON, catalog number: HE UHP1A)

  19. Pyridine (Sigma-Aldrich, catalog number: SHBK1583)

  20. N-tert-Butyldimethylsily-N-methyltrifluoroacetamide (TBDMS) (Sigma-Aldrich, catalog number: 394882)

  21. Ammonium-15N Chloride (15NH4Cl) (Sigma-Aldrich, catalog number: 229251)

  22. Amino Acid Standard mix (Sigma-Aldrich, catalog number: AAS18-5 ml)

  23. 10× Phosphate Buffered Saline (PBS) (Fisher Chemical, catalog number: 10010049)

  24. Hank's Balanced Salt Solutions (HBSS) (Fisher Chemical, catalog number: 14170112)

  25. EDTA (Fisher Chemical, catalog number: 6N011324)

  26. 15NH4Cl- solution (see Recipes)

  27. Extraction buffer (see Recipes)

  28. Internal Standard (see Recipes)

  29. Methoxyamine mix (see Recipes)

Equipment

  1. Dumont Tweezer, Style 5 (Electron Microscopy Sciences, catalog number: 0108-5-PO)

  2. -20 °C refrigerator

  3. Dissecting microscope (Zeiss, model: Stemi 2000-C)

  4. FirstHand Surgical Instrument Kits for Mice and Rats (Kent Scientific Corporation, model: INSMOUSEKIT)

  5. Battery-Operated Pestle Motor Mixer (Argos Technologies, catalog number: EW-44468-25)

  6. Omni Tissue Homogenizer (115V 125W) (OMNI International the Homogenizer Company, THB-01)

  7. Gas chromatograph-mass spectrometer (Agilent Technologies, model: 7890B/5977B GC-MS)

  8. DB-5ms GC Column (length 30 m, id 0.25 mm, film thickness 0.25 μm) (Agilent Technologies, catalog number: 122-5532)

  9. Gel Pump (Savant Instruments, GP110)

  10. Speed vac Plus (Savant Instruments, SC110A)

  11. Centrifuge (Eppendorf, catalog number: 5424)

  12. Thermomixer (Eppendorf ThermoMixer C, 5382000015)

Software

  1. MassHunter Workstation Software (Agilent Version B.07.01)

  2. MS Quantitation software (Agilent Version B.07.01/Build 7.1.524.0)

  3. Python 2.6+ (http://www.pythonxy.com)

  4. IsoCor Software (http://metasys.insa-toulouse.fr/software/isocor)

Procedure

Figure 1 is an overview of the procedures in this protocol.



Figure 1. The flow chart for this protocol. Animals are injected with the 15N tracer, and metabolites are extracted to analyze for 15N-labeled metabolites using GC MS.



  1. Tracer Injection

    1. Freshly prepare 15NH4Cl in PBS (see Recipe 1).

    2. Weigh mice and calculate the volume for the injection.

    3. Intraperitoneal injection (IP) of 15NH4Cl at 167 mg/kg or the same volume of PBS.


  2. Tissue collection

    1. Mice were quickly sacrificed with cervical dislocation at different time points after injection. For example, 0 min, 5 min, 15 min, 30 min and 60 min.

      Note: Different metabolites can reach their peak enrichment at different time points depending on the tissue.

    2. Enucleate mouse eyes and isolate neural retina and the eyecups under dissecting microscope in cold HBSS. Store the neural retina in a pre-weighed 1.5 ml microtube and the eyecup in a pre-weighted 2 ml microtube. Weigh the tissues and snap-freeze in liquid nitrogen.

    3. Perform cardiac puncture to withdraw 100-300 µl blood using a syringe with a 26G needle. Gently dispense the blood to EDTA-containing tubes. Place the blood samples on ice. Centrifuge the blood at 1,008 × g for 10 min at 4 °C, transfer the supernatant into a new 1.5 ml tube, and store supernatant in -20 °C refrigerator.

    4. Quickly remove brain and liver tissue, store them in a pre-weighed 2 ml microtube and snap-freeze them in liquid nitrogen.

      Note: Take out the whole brain to avoid heterogeneity from different regions. Cut a small piece of liver tissue (~40 mg) from the same lobe with scissors.


  3. Metabolite Extraction

    1. Tissue metabolite extraction (Figure 2)



      Figure 2. A schematic for metabolite extraction and derivatization. A. The brain, liver and eyecups were homogenized with an Omni THb Homogenizer, while the retina was homogenized with a pestle motor mixer. The homogenates were left on dry ice for 30 min and then centrifuged at 25,200 × g at 4 °C for 15 min. The supernatant was transferred to a glass insert containing 5 µl internal standard and dried with a speed vacuum. B. Add 10 µl of freshly prepared methoxyamine (20 mg/ml) into the dried samples in the insert and incubate for 90 min at 37 °C in a thermomixer, followed by a 30 min incubation at 70 °C after the addition of 30 µl of TBDMS. Transfer each insert into vials for GC MS analysis.


      1. Pre-chill extraction buffer (Recipe 2) on dry ice for 10 min.

      2. Transfer pre-chilled extraction buffer into tubes with tissues. Homogenize neural retina with a handheld pestle motor mixer in 140 µl extraction buffer for 15-20 s; Homogenize eyecup with an Omni THb Homogenizer in 200 µl extraction buffer for 15-20 s; Homogenize brain or liver tissues with the Omni THb Homogenizer for 20-30 s in extraction buffer (add 200 µl extraction buffer for every 5 mg tissues).

        Note: Change the disposable pestle for each sample. Clean the probe of Omni Thb homogenizer at least twice with clean water and wipes between samples to avoid cross-contamination. Leave the samples on dry ice for 30 min.

      3. Centrifuge the samples at 25,200 × g at 4 °C for 15 min.

      4. Filter the supernatants with a 0.2 µm syringe filter.

      5. Add 5 µl internal standard (Recipe 3) to each glass insert in 1.5 ml tube.

      6. Transfer the supernatant to each glass insert, open the tube lid to dry in a Speed vac in the cold room.

        Note: To ensure high sensitivity without overloading, transfer all the supernatant from retina or eyecup samples to dry and transfer 50 µl of brain or liver tissues to dry. Make sure the samples were fully dried. The moisture can affect the efficiency in derivatization and ionization.

    2. Plasma metabolite extraction

      1. Mix 10 µl plasma sample with 40 µl pre-chilled extraction buffer.

      2. Leave the mixture on ice for 15 min.

      3. Centrifuge the samples and transfer 10 µl into inserts with internal standard as described in tissue metabolite extraction.


  4. Sample preparation (Figure2)

    1. Freshly prepare methoxyamine mix (Recipe 4) and add 10 µl to each insert with the dried sample inside a 1.5 ml microtube. Mix and close the lid, then incubate the tube at 37 °C and 300 RPM for 90 min in a thermomixer.

      Note: Gently tap the tubes with inserts inside 3-5 times to mix, spin the samples down and close the lid tightly.

    2. After incubation, quickly spin the tubes and add 30 µl TBDMS to each sample. Incubate at 70 °C for 30 min.

    3. Spin down the tubes and transfer each insert into GC MS glass vials.


  5. GC-MS Analysis

    1. Pre-run preparation

      1. Install GC MS with DB-5 MS column and syringe needle.

      2. Set up a flow rate of helium gas at 1 ml/min.

      3. Fill the needle washing solvent vials A and B with hexane and methylene chloride respectively.

      4. Set up the GC oven temperature gradient as Table 1. The total run for each sample takes 31.5 min.


        Table 1. The parameters for GC oven temperature


      5. Set up the parameters for GC MS scan range, speed, frequency, cycle time and step size as Table 2. Choose the solvent delay for 5.4 min.


        Table 2. The parameters for GC MS scan range and speeder parameters on GC-MS



      6. Set up parameters for selection monitoring (SIM) mode as Table 3 for the ions of each metabolite that will be monitored.


        Table 3. List of ions for metabolites that are monitored under SIM mode


        Note: Isotopologuesare named as M0, M1, M2. M0 is the mass without labeling, and 1 to 2 represents the mass shift from the isotope labeling.


      7. Set up injection volume as 1 µl of the sample in split-less mode.

      8. Save these parameters as “GC MS nitrogen method”.

      9. Tune GC MS with autotune to check mass accuracy and ensure no leakage in the system.

      Note: Air leakage and dirty source in the system can significantly decrease the sensitivity. It is critical to tune the system weekly with regular replacement of the air trap and source maintenance.

    2. Run sample

      1. Place samples in the auto-sampler.

      2. Fill the sequence table for sample names and vial names. Select GC MS nitrogen method.

      3. Run samples with GC MS.

        Note: Check the instrument that it runs properly, especially for the long run. The typical instrument running failure includes an improperly filled sequence table, defective syringe needle and malfunctional filament.

    3. GC MS data processing

      1. Set up a quantitation method using Agilent MS Quantitation software based on Table 3.

      2. Click “File” in the software to set up a new batch.

      3. Select the quantitation method and extract the peak area for each selected ion.

      4. Export the peak area into an excel file.


  6. Nature abundance correction

    1. Nature abundance correction

      1. Install IsoCor for natural abundance correction software

        Download IsoCor software at http://metasys.insa-toulouse.fr/software/isocor; Download Python 2.6+ (http://www.pythonxy.com) and install modules: wxPython (v 2.8.11.0), NumPy( v1.6.0.2), SciPy( v0.9.0.1).

      2. Set up parameters for IsoCor software

      3. Edit “Metabolites. dat” file. Open the “Metabolites.dat” file under the IsoCor folder with Notepad and input metabolites and their elemental formula in Table 4. Save the file in the same folder.


        Table 4. List of metabolites and their elemental formula for natural abundance correction



      4. Edit “Derivatives. dat” file. Using Notepad to edit this file and input the chemical derivatives with their elemental formulas as Table 5. Set up the input data file as Table 6 and copy the GC MS peak area (ion intensity) into this table. Save the file in the same folder.


        Table 5. List of chemical derivatives with their elemental formula


        TBDMS1, 2 or 3 represents the number of TBDMS in the derivative metabolite.


    2. Data correction

      1. Save the Excel Input data file (Table 6) as “xxx. txt.”

      2. Open IsoCor software and select Isotopic tracer as “N”.

      3. Select the purity of the tracer as (0.02; 0.98).

        Note: The purity is dependent on the tracer you are using. For example, the purity of 15NH4Cl is 98%. It is represented as (0.02; 0.98).

      4. Load the input data file using the “Load multiple means” button.

      5. Click on the “Process” button.

      6. Open a new Excel file and load Input data file_res.txt.

        Note: The output of the calculations is automatically saved in a .txt file as (Input File_res.txt) and (Input File_log.txt). Save them in the same folder.Table 7 is a representative output file after natural abundance correction.


        Table 6. Template of Input data file for IsoCor

        The intensity is representative data from the extracted peak area for specifically monitored ion.

Data analysis

Representative data



Figure 3. GC MS chromatogram from standards and mouse tissue samples. A. GC MS chromatogram for M0 glutamate (m/z 432.3) from an amino acid standard mix. The calibration curve for M0 glutamate was calculated using the standard mix. B. The chromatogram of M0 glutamate (m/z 432.3) was extracted from the TIC in mouse retina sample received PBS injection.


Figure 3 is the representative GC MS chromatogram from standards and mouse tissule samples. Table 7 is the representative GC MS data that after natural abundance correction from liver tissue after injection with 15NH4Cl. Isotopologue distribution is the enrichment of 15N from the tracer. Except for branch chain amino acids including leucine, isoleucine, and valine, all the other metabolites reach their peak enrichment at 5 min after single tracer injection. The enrichment drops at 15 min due to metabolic degradation.


Table 7. The representative GC MS data of isotopologue distribution of liver tissue at 5 min and 15 min after natural abundance correction Isotopogue distribution

Recipes

  1. 15NH4Cl- solution

    Weigh ammonium-15N Chloride and dissolve in filtered 1× PBS at 33 mg/ml

  2. Extraction buffer

    Mix methanol and HPLC water at 80:20 (Vol:Vol)

  3. Internal Standard

    Weigh myristic acid-D27 powder and dissolve in Isopropanol: methanol: HPLC water mixture at 2:5:2 ratio (Vol:Vol:Vol)

  4. Methoxyamine mix

    Dissolve methoxyamine hydrochloride in pyridine solution at 20 mg/ml

    Note: Take the pyridine solution with a syringe needle through the sealed rubber lid to avoid moisture.

Acknowledgments

National Institutes of Health Grant EY026030 and EY031324 (To JD), BrightFocus Foundation (To JD), and the Retina Research Foundation (To JD) supported this work.

Competing interests

The authors declare no conflicts of interest.

Ethics

Mouse experiments were performed in accordance with the National Institutes of Health guidelines and the protocol (#1611004455, 01/18/2020-01/17/2023) was approved by the Institutional Animal Care and Use Committee of West Virginia University.

References

  1. Albertino, B., Borgialli, R., Guerra, M. G., Gasparri, G., Oliaro, A. and Dei Poli, M. (1989). Sequential study of the immunologic picture in neoplasm patients before and after surgical intervention. Minerva Chir 44(17): 1917-1920.
  2. Chokkathukalam, A., Kim, D. H., Barrett, M. P., Breitling, R. and Creek, D. J. (2014). Stable isotope-labeling studies in metabolomics: new insights into structure and dynamics of metabolic networks. Bioanalysis 6(4): 511-524.
  3. Du, J., Linton, J. D. and Hurley, J. B. (2015). Probing Metabolism in the Intact Retina Using Stable Isotope Tracers. Methods Enzymol 561: 149-170.
  4. Du, J., Yanagida, A., Knight, K., Engel, A. L., Vo, A. H., Jankowski, C., Sadilek, M., Tran, V. T., Manson, M. A., Ramakrishnan, A., Hurley, J. B. and Chao, J. R. (2016). Reductive carboxylation is a major metabolic pathway in the retinal pigment epithelium. Proc Natl Acad Sci U S A 113(51): 14710-14715.
  5. Fiehn, O. (2002). Metabolomics--the link between genotypes and phenotypes. Plant Mol Biol 48(1-2): 155-71.
  6. Fiehn, O. (2016). Metabolomics by Gas Chromatography-Mass Spectrometry: Combined Targeted and Untargeted Profiling. Curr Protoc Mol Biol 114: 30-34 31-30 34 32.
  7. Grenell, A., Wang, Y., Yam, M., Swarup, A., Dilan, T. L., Hauer, A., Linton, J. D., Philp, N. J., Gregor, E., Zhu, S., Shi, Q., Murphy, J., Guan, T., Lohner, D., Kolandaivelu, S., Ramamurthy, V., Goldberg, A. F. X., Hurley, J. B. and Du, J. (2019). Loss of MPC1 reprograms retinal metabolism to impair visual function. Proc Natl Acad Sci U S A 116(9): 3530-3535.
  8. Jang, C., Chen, L. and Rabinowitz, J. D. (2018). Metabolomics and Isotope Tracing. Cell 173(4): 822-837.
  9. Wang, Y., Bai, C., Ruan, Y., Liu, M., Chu, Q., Qiu, L., Yang, C. and Li, B. (2019). Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia. Nat Commun 10(1): 201.
  10. Xu, R., Ritz, B. K., Wang, Y., Huang, J., Zhao, C., Gong, K., Liu, X. and Du, J. (2020). The retina and retinal pigment epithelium differ in nitrogen metabolism and are metabolically connected. J Biol Chem 295(8): 2324-2335.
  11. Yam, M., Engel, A. L., Wang, Y., Zhu, S., Hauer, A., Zhang, R., Lohner, D., Huang, J., Dinterman, M., Zhao, C., Chao, J. R. and Du, J. (2019). Proline mediates metabolic communication between retinal pigment epithelial cells and the retina. J Biol Chem 294(26): 10278-10289.
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Copyright: © 2021 The Authors; exclusive licensee Bio-protocol LLC.
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Xu, R., Wang, Y. and Du, J. (2021). Tracing Nitrogen Metabolism in Mouse Tissues with Gas Chromatography-Mass Spectrometry . Bio-protocol 11(4): e3925. DOI: 10.21769/BioProtoc.3925.
  2. Xu, R., Ritz, B. K., Wang, Y., Huang, J., Zhao, C., Gong, K., Liu, X. and Du, J. (2020). The retina and retinal pigment epithelium differ in nitrogen metabolism and are metabolically connected. J Biol Chem 295(8): 2324-2335.
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