Thank you very much for dealing with our protocol issued on March 5, 2018 (https://bio-protocol.org/e2751).

We have noticed a simple, but a very problematic typo in a recipe of physiological saline in the protocol. In the Recipe section of the protocol, the concentration of CaCl₂ is "8 mM" as below, but this is completely wrong. Correct concentration of CaCl₂ is "1.8 mM". We are sorry that we had not noticed this typo when we submitted a manuscript. We would appreciate it if you could make a correction only to this part ("8" --> "1.8").

References

Tomina, Y. and Wagenaar, D. A. (2018). Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia. Bio-protocol 8(5): e2751.