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A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila   

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Original research article

A brief version of this protocol appeared in:
eLIFE
Oct 2017

Abstract

Inducing an injury specifically to Drosophila flight muscles is a difficult task, owing to the small size of the muscles and the presence of the cuticle. The protocol described below provides an easy and reproducible method to induce injury in the Drosophila flight muscles.

Keywords: Drosophila flight muscles, Injury, Regeneration, Insect satellite cells

Background

Muscles in vertebrates undergo regeneration, a process attributed to the Satellite cells, the resident stem cells. Our lab has recently shown that Drosophila flight muscles harbor stem cells similar to vertebrate satellite cells, namely insect satellite cells and show proliferation response to muscle injury (Chaturvedi et al., 2017). The ease of fly genetics and our method of inducing injury open up an opportunity to address relevant questions in the field of regenerative biology. We have standardized a protocol for injuring dorsal longitudinal muscles (DLMs) fibers with better precision, and this method can be used for investigating the repair mechanisms involved in muscles after the injury.

Copyright Chakraborty et al. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Chakraborty, K., VijayRaghavan, K. and Gunage, R. (2018). A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila. Bio-protocol 8(10): e2860. DOI: 10.21769/BioProtoc.2860.
  2. Chaturvedi, D., Reichert, H., Gunage, R. D. and VijayRaghavan, K. (2017). Identification and functional characterization of muscle satellite cells in Drosophila. Elife 6.
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