Abstract
Plant regeneration refers to a process through which an explant is differentiated to a whole plant under certain conditions. It has been shown that two plant hormones, auxin and cytokinin, play critical roles within this process (Duclercq et al., 2011). Cytokinin induces shoot regeneration, whereas auxin promotes root production. In addition to hormones, recent study has revealed that age cue serves as a common element behind plant cell totipotency (Toledano et al., 2012). Here we present an easy protocol to assess the shoot regenerative capacity of Arabidopsis and tobacco leaves of different ages.
Keywords: Shoot regeneration, Cytokinin, Auxin, Plant age
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The work in the Wang laboratory is supported by grants from the National Natural Science Foundation of China (31430013; 31222029; 912173023), the State Key Basic Research Program of China (2013CB127000), the Shanghai Outstanding Academic Leader Program (15XD1504100) and the NKLPMG Key Research Program. This protocol was adapted from our recent publications (Zhang et al., 2015a; Zhang et al., 2015b).
References
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