Abstract
The blood–brain barrier (BBB) is a highly selective permeability barrier that separates the circulating blood from the brain extracellular fluid in the central nervous system. The blood–brain barrier allows the passage of water, some gases, and lipid soluble molecules by passive diffusion, as well as the selective transport of molecules such as glucose and amino acids that are crucial to neural function. This protocol provides a full, detailed method for measuring blood-brain-barrier permeability of mice with Evans blue (EB), that leakage was used to assess blood-brain barrier (BBB) permeability (sample: Anterior Cingulate Cortex, ACC).
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This study was supported by grants from the National Natural Science Foundation of China (81070888 and 81230025 to J.-L.C., 81200859 to H.-L.D. 81202320 to Y. -B. G.), Natural Science Foundation of Jiangsu Education Department (13KJB320024 to J.-X.Y.), Natural Science Foundation of Jiangsu Province (BK2011198 to J.-L.C.), The Scientific Innovation Group of “Qing Lan Project” of Jiangsu Province (to J.-L.C.), and Jiangsu Provincial Special Program of Medical Science (BL2014029). We thank Wen-Zhen Duan, Heng Cai, and Tian-Hui Gao for administrative assistance.
References
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