TY  - JOUR
TI  - H2O2 Kill Assays of Biofilm Bacteria
AU  - Khakimova, Malika  
AU  - Nguyen, Dao  
VL  - 3
IS  - 21
PY  - 2013
DA  - 2013/11/05
SP  - e952
C1  - Bio-protocol 2013;3:e952
DO  - 10.21769/BioProtoc.952
UR  - https://doi.org/10.21769/BioProtoc.952
AB  - Ubiquitous in nature and often surface associated, biofilms cause numerous chronic human infections. Biofilms are structured multicellular bacterial communities where cells are entrapped in a polymer matrix. Bacteria growing as biofilms are characterized by marked tolerance to many biocides, including oxidants such as hydrogen peroxide. Hydrogen peroxide is both produced by host phagocytic cells, and used as an antimicrobial compound. Understanding biofilm tolerance to hydrogen peroxide is therefore relevant to the persistence of Pseudomonas aeruginosa in human infections (such as chronic Pseudomonas aeruginosa infections in cystic fibrosis airways) as well as in environmental settings (such as water pipes). This protocol was developed to determine the tolerance of Pseudomonas aeruginosa biofilms to hydrogen peroxide (H2O2) killing. The bacteria are grown as colony biofilms on polycarbonate membranes, as previously described in Walters et al. 2003. The protocol may be adapted for other bacterial, with appropriate changes in H2O2 concentrations, since different bacterial species may be more or less susceptible to H2O2 than Pseudomonas aeruginosa.
KW  - Pseudomonas aeruginosa
KW  - Biofilms
KW  - Oxidative stress
KW  - Hydrogen peroxide
KW  - Tolerance
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False