TY  - JOUR
TI  - Flow Cytometric Analysis of Calcium Influx Assay in T cells
AU  - Lee, Sun-Hwa 
VL  - 3
IS  - 18
PY  - 2013
DA  - 2013/09/20
SP  - e910
C1  - Bio-protocol 2013;3:e910
DO  - 10.21769/BioProtoc.910
UR  - https://doi.org/10.21769/BioProtoc.910
AB  - Calcium influx is one of the key signaling events upon stimulation of T cell receptors (TCR) and plays an important role for T cell activation, proliferation, and differentiation. Phorbol myristate acetate (PMA) and calcium ionophore ionomycin are commonly utilized as stimulants in a variety of immunologic assays including T cell activation. PMA is a protein kinase C (PKC) activator, resulting in the activation of Ras, a small GPTase. When PMA and ionomycin are used together, TCR signaling downstream of PKC and Ras can be activated without activation of TCR-triggerd signaling events. This protocol describes the flow cytometry analysis of intracellular calcium influx in T cells stimulated with PMA and ionomycin.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False