TY  - JOUR
TI  - Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes
AU  - Xia, Ai  
AU  - Peery, Ashley  
AU  - Kamali, Maryam  
AU  - Liang, Jiangtao  
AU  - Sharakhova, Maria V. 
AU  - Sharakhov, Igor V.
VL  - 3
IS  - 16
PY  - 2013
DA  - 2013/08/20
SP  - e860
C1  - Bio-protocol 2013;3:e860
DO  - 10.21769/BioProtoc.860
UR  - https://doi.org/10.21769/BioProtoc.860
AB  - Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This protocol was used to map genes (Sharakhova et al., 2010) and microsatellite markers (Kamali et al., 2011; Peery et al., 2011) on polytene chromosomes from ovarian nurse cells and salivary glands of malaria mosquitoes. Detailed physical genome mapping performed on polytene chromosomes has the potential to link DNA sequences to specific chromosomal structures such as heterochromatin (Sharakhova et al., 2010). This method also allows comparative cytogenetic studies (Sharakhova et al., 2011; Xia et al., 2010), and reconstruction of species phylogenies (Kamali et al., 2012).
KW  - Mosquito
KW  - Chromosome
KW  - Mapping
KW  - Genome
KW  - FISH
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False