TY  - JOUR
TI  - Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue
AU  - Wilting, Saskia M.
AU  - Steenbergen, Renske DM
VL  - 3
IS  - 15
PY  - 2013
DA  - 2013/08/05
SP  - e848
C1  - Bio-protocol 2013;3:e848
DO  - 10.21769/BioProtoc.848
UR  - https://doi.org/10.21769/BioProtoc.848
AB  - As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the accurate determination of gene/microRNA expression. Laser capture microdissection enables the researcher to obtain homogeneous ultrapure cell selections from heterogeneous starting material. The following protocol was optimized for the isolation of total RNA from cervical (premalignant) squamous epithelial cells from fresh frozen biopsy specimens.
KW  - LCM
KW  - RNA
KW  - Fresh frozen
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False