TY  - JOUR
TI  - Whole Spleen Flow Cytometry Assay
AU  - Yam, Cathy S.
AU  - Hajjar, Adeline M.
VL  - 3
IS  - 15
PY  - 2013
DA  - 2013/08/05
SP  - e834
C1  - Bio-protocol 2013;3:e834
DO  - 10.21769/BioProtoc.834
UR  - https://doi.org/10.21769/BioProtoc.834
AB  - In the Whole Spleen Flow Cytometry Assay, we used splenocytes directly ex vivo for stimulation with a variety of TLR ligands. The splenocytes were stimulated for a total of 4 hours, then stained for intracellular cytokines. We then examined cytokine production via flow cytometry. This allowed us to compare the responses of minimally manipulated primary macrophages/monocytes and conventional dendritic cells.
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False