TY  - JOUR
TI  - MACS Isolation and Culture of Mouse Liver Mesothelial Cells
AU  - Li, Yuchang 
AU  - Lua, Ingrid 
AU  - Asahina, Kinji 
VL  - 3
IS  - 13
PY  - 2013
DA  - 2013/07/05
SP  - e815
C1  - Bio-protocol 2013;3:e815
DO  - 10.21769/BioProtoc.815
UR  - https://doi.org/10.21769/BioProtoc.815
AB  - Mesothelial cells (MCs) form a single squamous epithelial cell layer and cover the surfaces of the internal organs, as well as the walls of cavities. The isolation of MCs is of great importance to study their function and characteristics for the understanding of physiology and pathophysiology of the liver. Glycoprotein M6a (GPM6A) was originally identified as a cell surface protein expressed in neurons and recently its expression was reported in epicardium and liver MCs (Wu et al., 2001; Bochmann et al., 2010; Li et al., 2012). Here we describe a method to isolate MCs from the adult mouse liver with anti-GPM6A antibodies. Under the low glucose and serum concentration, primary MCs grow and form epithelial colonies (Figure 1). Figure 1.  Liver MCs 2 days in culture (20x objective)
KW  - Antibody
KW  - Epithelial-mesenchymal transition
KW  - Fibrosis
KW  - Gpm6a
KW  - Pronase
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False