TY  - JOUR
TI  - Metabolic Labeling of Yeast RNA with Radioactive Uracil
AU  - Wei, Yuehua 
VL  - 3
IS  - 7
PY  - 2013
DA  - 2013/04/05
SP  - e623
C1  - Bio-protocol 2013;3:e623
DO  - 10.21769/BioProtoc.623
UR  - https://doi.org/10.21769/BioProtoc.623
AB  - To examine gene expression, Northern blot or Real-Time PCR can be used to detect low abundant RNA such as mRNA. However, for high abundant RNAs such as rRNA and tRNA, Northern blot will not be able to discriminate the newly synthesized RNA from total RNA. Therefore, metabolic labeling is necessary to evaluate the expression of rRNA and tRNA genes. In this protocol, I describe a step-by-step method for labeling yeast RNA with radioactive uracil and examine the synthesis of these high abundant RNAs.
KW  - Autoradiography
KW  - Metabolic labeling
KW  - Tritium
KW  - RRNA
KW  - TRNA
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False