TY  - JOUR
TI  - Assessment of Saccharomyces cerevisiae Survival Upon Exposure to Transient High Pressure and Temperature in a High-Intensity Shock Tube for Astrobiology (HISTA)
AU  - Dhage, Riya 
AU  - Roy, Arijit 
AU  - Sivaraman, Bhalamurugan 
AU  - Rajyaguru, Purusharth I
VL  - 16
IS  - 14
PY  - 2026
DA  - 2026/07/20
SP  - e5766
C1  - Bio-protocol 2026;16:e5766
DO  - 10.21769/BioProtoc.5766
UR  - https://doi.org/10.21769/BioProtoc.5766
AB  - Understanding microbial survival under extreme planetary conditions is critical for astrobiology and stress biology. Several experimental platforms, including radiation, desiccation, and microgravity, have been used to mimic extraterrestrial environments; however, controlled simulation of high-intensity shock waves has not been used to assess microbial survival. Here, we describe a detailed protocol for shock processing of Saccharomyces cerevisiae using the high-intensity shock tube for astrochemistry (HISTA), which generates high-Mach-number shock waves under inert gas conditions. Yeast cells are drop-casted onto a metal flange, exposed to transient high-pressure shock waves, and recovered for downstream survival and cellular analyses. Shock intensity can be precisely tuned by adjusting driver pressure, diaphragm thickness, and driven gas pressure. This protocol provides a platform to investigate microbial adaptation to shock waves.
KW  - Saccharomyces cerevisiae
KW  - Shock tube
KW  - Shock waves
KW  - Yeast survival
KW  - HISTA
KW  - Mars
KW  - Stress conditions
KW  - Astrobiology
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False