TY  - JOUR
TI  - A Protocol to Induce Brown and Beige Adipocyte Differentiation From Murine and Human Adipose-Derived SVF
AU  - Yadav, Rohit Raj
AU  - Mishra, Nandita 
AU  - Verma, Narendra 
VL  - 15
IS  - 23
PY  - 2025
DA  - 2025/12/05
SP  - e5522
C1  - Bio-protocol 2025;15:e5522
DO  - 10.21769/BioProtoc.5522
UR  - https://doi.org/10.21769/BioProtoc.5522
AB  - Adipose cells vary functionally, with white adipocytes storing energy and brown/beige adipocytes generating heat. Mouse and human subcutaneous white adipose tissue (WAT)-derived stromal vascular fraction (SVF) provides mesenchymal stem cells (MSCs) that can be differentiated into thermogenic adipocytes using pharmacological cocktails. After six days of browning induction, these cells exhibited significant upregulation of thermogenic markers (UCP1, Cidea, Dio2, PRDM16) along with adipogenic genes (PPARγ, aP2), showing enhanced thermogenic potential. This in vitro system offers a practical platform to study adipogenesis and thermogenic regulation.
KW  - Adipocyte differentiation
KW  - Thermogenesis
KW  - SVF
KW  - Beige adipocytes
KW  - Brown adipocytes
KW  - MSCs
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False