TY  - JOUR
TI  - Intracerebral Cannula Implantation in Mouse: A Proposed Method to Assess Glioblastoma Invasiveness and Serial Locoregional Treatment
AU  - Henry, Aurélie 
AU  - Stordeur, Philippe 
AU  - Lapierre, Andrée 
AU  - Buttenaers, Chloé 
AU  - Lefevre, Marc 
AU  - Noelanders, Maëlle 
AU  - Mottart, Cathy 
AU  - Delincé, Lucie 
AU  - Destiné, Simon 
AU  - Etienne, Quentin  
AU  - Nijskens, Caroline  
AU  - Filee, Patrice 
AU  - Thirion, Gaëtan 
VL  - 15
IS  - 22
PY  - 2025
DA  - 2025/11/20
SP  - e5503
C1  - Bio-protocol 2025;15:e5503
DO  - 10.21769/BioProtoc.5503
UR  - https://doi.org/10.21769/BioProtoc.5503
AB  - Research on brain disorders, particularly in the field of oncology, requires in vivo models to evaluate various therapeutic approaches, including intracerebral drug delivery. To meet this requirement, the implantation of intracerebral cannulas offers a reliable method for administering candidate therapeutics directly into the brain. This protocol describes a surgical technique for cannula implantation in mice, enabling repeated administration of therapeutic compounds in the context of glioblastoma treatment. The method was designed with an emphasis on using accessible, easy-to-handle, and sterilized tools to optimize surgical outcomes. Particular attention was also given to animal welfare, notably through refined procedures for asepsis, anesthesia, and postoperative care.
KW  - Cannula
KW  - Intracerebral
KW  - Tumor
KW  - Infusion
KW  - Cement
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False