TY  - JOUR
TI  - A Novel Composite Method of Post-stroke Epilepsy Induction
AU  - Guo, Yiting 
AU  - Cheung, Raymond Tak Fai
VL  - 15
IS  - 14
PY  - 2025
DA  - 2025/07/20
SP  - e5387
C1  - Bio-protocol 2025;15:e5387
DO  - 10.21769/BioProtoc.5387
UR  - https://doi.org/10.21769/BioProtoc.5387
AB  - The global burden of stroke has increased in the past several decades, and post-stroke epilepsy (PSE) is a common complication. Contrasted with the advancement in knowledge of stroke pathophysiology, the exact pathogenesis of PSE is unclear. Various animal stroke models have been utilized to investigate the underlying mechanisms of PSE, but the success rate of PSE induction is low. To address this limitation, a novel PSE model was established in the rat by inducing status epilepticus using lithium-pilocarpine one week after photothrombotic stroke. Successful indication of status epilepticus and mortality rate at three days after status epilepticus were the main measurements. Potential usefulness of this model was also illustrated by preliminary results on locomotor activity, exploratory behavior, and anxiety level evaluated using the open-field test, as well as mossy fiber sprouting (MFS) in the hippocampal dentate granule cells using Zinc transporter 3 immunofluorescence staining at 8 weeks after PSE induction. This novel composite method of PSE induction may facilitate future studies on the pathogenesis and treatment of PSE.
KW  - Post-stroke epilepsy
KW  - Photothrombosis
KW  - Epilepsy
KW  - Open field
KW  - Mossy fiber sprouting
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False