TY  - JOUR
TI  - Isolation and Characterization of Cervical Cancer-Associated Mesenchymal Stem Cells From Primary Tumors Using Explant Culture
AU  - Singla, Surbhi 
AU  - Khurana, Sanchita 
AU  - Bagga, Rashmi 
AU  - Srinivasan, Radhika 
AU  - Bhattacharyya, Shalmoli 
VL  - 15
IS  - 12
PY  - 2025
DA  - 2025/06/20
SP  - e5358
C1  - Bio-protocol 2025;15:e5358
DO  - 10.21769/BioProtoc.5358
UR  - https://doi.org/10.21769/BioProtoc.5358
AB  - Cancer-associated mesenchymal stem cells (Ca-MSCs), an integral part of the tumor microenvironment, play a major role in modulating tumor progression; they have been reported to progress as well as inhibit various cancers, including cervical cancer. To understand the exact role of Ca-MSCs in tumor modulation, it is necessary to have an optimized protocol for Ca-MSCs isolation. This work demonstrates the isolation and expansion of a primary culture of cervical cancer–associated MSCs (CCa-MSCs) from the biopsy sample of cervical cancer patients using the explant culture technique. The isolated cells were characterized according to International Society for Cellular Therapy (ISCT) guidelines. Morphological analysis revealed that cells were adherent to the plastic surface and possessed spindle-shaped morphology. Flow cytometry analysis of the cells showed high expression (~98%) for MSC-specific cell surface markers (CD90, CD73, and CD105), negative expression (
KW  - Mesenchymal stem cells
KW  - Cancer-associated MSCs
KW  - Cervical cancer
KW  - Explant culture
KW  - Trilineage differentiation
KW  - Adipogenic
KW  - Chondrogenic
KW  - Osteogenic
KW  - Tumor microenvironment
KW  - Cancer stem cells
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False