TY  - JOUR
TI  - Surgical Implantation of a Telemetry-Based Pressure Sensor in the Internal Jugular Vein to Monitor Respiration Wirelessly
AU  - Kushwaha, Neha 
AU  - Dasgupta, Debanjan 
VL  - 15
IS  - 12
PY  - 2025
DA  - 2025/06/20
SP  - e5343
C1  - Bio-protocol 2025;15:e5343
DO  - 10.21769/BioProtoc.5343
UR  - https://doi.org/10.21769/BioProtoc.5343
AB  - Active sampling, such as respiration, is known to play a major role in modulating how sensory information is perceived and encoded in the field of olfaction. Hence, monitoring respiration is crucial for understanding olfactory-guided behavior and physiology. Several methods used to measure respiration, such as infrared cameras, piezoelectric sensors, video monitoring, temperature probes, intubation, and intranasal cannula, require the animal or at least its head to be fixed. However, telemetry-based sensors can be used wirelessly, allowing animals to move freely. Here, we describe the surgical protocol to implant a telemetry pressure sensor in the internal jugular vein to detect changes in thoracic pressure. The sensor can thus help in monitoring respiration by transmitting the signal wirelessly. We describe a way of inserting the probe into the right jugular vein aseptically while housing the transmitter underneath the skin on the back of the animal. Next, based on the optimal spot for the best signal, we secure the position of the probe and suture the skin. The animal then undergoes regular post-operative care with painkillers and soft diets for up to a week. The method offers two main advantages; first, it uses a strategy similar to the jugular vein catheterization, which is widely established in rodents. Second, it minimizes the need for extensive post-operative care, including not having to shift to a liquid diet post-recovery. This makes the animals fit for most behavioral experiments requiring water or food restrictions.
KW  - Wireless respiration recording
KW  - Telemetry-based pressure sensor
KW  - Aseptic surgery
KW  - Catheterization of the internal jugular vein
KW  - Thoracic cavity
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False