TY  - JOUR
TI  - Development of Polyethylene Glycol Diacrylate-Based Micropattern Substrate to Study the Interplay Between Surface Topography and Cellular Response for Tissue Engineering Applications
AU  - Darwis, Mohd Khairul Akma
AU  - Levario-Diaz, Victoria 
AU  - Pashapour, Sadaf 
AU  - Voigt, Jonah Luka
AU  - Lebaudy, Eloïse 
AU  - Sabani, Norhayati 
AU  - Bakar, Ahmad Shuhaimi Abu
AU  - Vrana, Nihal Engin
AU  - Lavalle, Philippe 
AU  - Cavalcanti-Adam, Elisabetta Ada
AU  - Ngalim, Siti Hawa
VL  - 15
IS  - 10
PY  - 2025
DA  - 2025/05/20
SP  - e5323
C1  - Bio-protocol 2025;15:e5323
DO  - 10.21769/BioProtoc.5323
UR  - https://doi.org/10.21769/BioProtoc.5323
AB  - A key goal in the bioengineering field is the development of surface patterning of proteins that guide and control cellular organization. To this end, we developed a method to create a microstructured hydrogel based on soft-lithography techniques using polydimethylsiloxane (PDMS) and polyethylene glycol diacrylate (PEGDA). This approach involves the design of microfluidic geometries using graphical software, employing PDMS as a mold and leaving PEGDA as a substrate for the fabrication of microstructures and, thus, patterning extracellular matrix (ECM) proteins to promote cell adhesion. The combination of these techniques allows the fabrication of hydrogel microstructures without following conventional photolithography methods, such as the use of a photomask, the alignment required to produce the patterns, and the associated expenses. This study highlights the versatility and potential of PEGDA-based hydrogels as platforms to advance tissue engineering strategies.
KW  - PEGDA
KW  - Micropatterns
KW  - PDMS
KW  - Protein patterning
KW  - Biomaterials
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False