TY  - JOUR
TI  - A Robust and Easy Protein Purification Method Using SpyDock-Modified Resin
AU  - Yang, Xiaofeng 
AU  - Lin, Zhanglin 
AU  - Xiang, Ya 
AU  - Chen, Binrui 
AU  - Lao, Zisha 
VL  - 15
IS  - 8
PY  - 2025
DA  - 2025/04/20
SP  - e5270
C1  - Bio-protocol 2025;15:e5270
DO  - 10.21769/BioProtoc.5270
UR  - https://doi.org/10.21769/BioProtoc.5270
AB  - Protein purification is a critical step in both life sciences and biomanufacturing. Traditional affinity chromatography (AC) methods, including His-tag-based purification, provide high-purity proteins but are limited by the high cost of resins and the need for additional tag-removal steps. In this protocol, we present a reusable SpyDock-modified epoxy resin coupled with a pH-inducible self-cleaving intein for direct purification of proteins with authentic N-termini. This method enables efficient protein purification from cell lysates, achieving high purity (>90%) and yields comparable to the His-tag approach, without requiring tag removal. The SpyDock-modified resin protocol is robust, easy to implement, and cost-effective, making it suitable for both research and large-scale industrial applications.
KW  - Protein purification
KW  - Protein immobilization
KW  - Spy chemistry
KW  - SpyDock-modified epoxy resin
KW  - Intein
KW  - Authentic N-termini
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False