TY  - JOUR
TI  - HS–GC–MS Method for the Diagnosis of IBD Dynamics in a Model of DSS-Induced Colitis
AU  - Shagaleeva, Olga Yu.
AU  - Kashatnikova, Daria A.
AU  - Kardonsky, Dmitry A.
AU  - Efimov, Boris A.
AU  - Ivanov, Viktor A.
AU  - Smirnova, Svetlana V.
AU  - Zorkina, Yana A.
AU  - Vorobjeva, Elizaveta A.
AU  - Silantiev, Artemiy S.
AU  - Kazakova, Viktoriia D.
AU  - Kolesnikova, Irina V.
AU  - Markelova, Maria I.
AU  - Vanyushkina, Anna A.
AU  - Chaplin, Andrei V.
AU  - Grigoryeva, Tatiana V.
AU  - Zakharzhevskaya, Natalya B.
VL  - 15
IS  - 6
PY  - 2025
DA  - 2025/03/20
SP  - e5246
C1  - Bio-protocol 2025;15:e5246
DO  - 10.21769/BioProtoc.5246
UR  - https://doi.org/10.21769/BioProtoc.5246
AB  - Inflammatory bowel disease (IBD) is highly prevalent globally and, in the majority of cases, remains asymptomatic during its initial stages. The gastrointestinal microbiota secretes volatile organic compounds (VOCs), and their composition alters in IBD. The examination of VOCs could prove beneficial in complementing diagnostic techniques to facilitate the early identification of IBD risk. In this protocol, a model of sodium dextran sulfate (DSS)-induced colitis in rats was successfully implemented for the non-invasive metabolomic assessment of different stages of inflammation. Headspace–gas chromatography–mass spectrometry (HS–GC–MS) was used as a non-invasive method for inflammation assessment at early and remission stages. The disease activity index (DAI) and histological method were employed to assess intestinal inflammation. The HS–GC–MS method demonstrated high sensitivity to intestine inflammation, confirmed by DAI and histology assay, in the acute and remission stages, identifying changes in the relative content of VOCs in stools. HS–GC–MS may be a useful and non-invasive method for IBD diagnostics and therapy effectiveness control.
KW  - Headspace–gas chromatography–mass spectrometry (HS–GC–MS)
KW  - Volatile organic compounds (VOCs)
KW  - Metabolomic profile
KW  - IBD
KW  - DSS-induced colitis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False