TY  - JOUR
TI  - Culture and Characterization of Differentiated Airway Organoids from Fetal Mouse Lung Proximal Progenitors
AU  - Zhang, Zhonghui 
AU  - Tao, Chengxu 
AU  - Li, Qiuling 
VL  - 14
IS  - 23
PY  - 2024
DA  - 2024/12/05
SP  - e5129
C1  - Bio-protocol 2024;14:e5129
DO  - 10.21769/BioProtoc.5129
UR  - https://doi.org/10.21769/BioProtoc.5129
AB  - Developing a physiologically relevant in vitro model of the respiratory epithelium is critical for understanding lung development and respiratory diseases. Here, we describe a detailed protocol in which the fetal mouse proximal epithelial progenitors were differentiated into 3D airway organoids, which contain terminal-differentiated ciliated cells and basal stem cells. These differentiated airway organoids could constitute an excellent experimental model to elucidate the molecular mechanisms of airway development and epithelial cell fate determination and offer an important tool for establishing pulmonary dysplasia disease in vitro.
KW  - Airway organoids
KW  - Fetal
KW  - Proximal progenitors
KW  - Lung development
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False