TY  - JOUR
TI  - Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro
AU  - Parchure, Anup 
AU  - von Blume, Julia 
VL  - 14
IS  - 20
PY  - 2024
DA  - 2024/10/20
SP  - e5095
C1  - Bio-protocol 2024;14:e5095
DO  - 10.21769/BioProtoc.5095
UR  - https://doi.org/10.21769/BioProtoc.5095
AB  - Chromogranin B and other members of the granin protein family form condensates that recruit clients like proinsulin. The condensation in the lumen of trans-Golgi network (TGN) is critical for the biogenesis of secretory granules. Here, we describe a protocol to purify the tagged version of chromogranin B close to its native form at the TGN, which can then be utilized for microscopy-based assays to monitor condensate formation in vitro and client partitioning depending on the material properties of chromogranin B assemblies.
KW  - Phase separation
KW  - Chromogranin B
KW  - Protein purification
KW  - Client-partitioning
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False