TY  - JOUR
TI  - Protocol for Imaging the Same Class IV Neurons at Different Stages of Development
AU  - Shree, Sonal 
AU  - Howard, Jonathon 
VL  - 14
IS  - 16
PY  - 2024
DA  - 2024/08/20
SP  - e5052
C1  - Bio-protocol 2024;14:e5052
DO  - 10.21769/BioProtoc.5052
UR  - https://doi.org/10.21769/BioProtoc.5052
AB  - In this protocol, we focused on analyzing internal branches of Drosophila class IV neurons. These neurons are characterized by their highly branched axons and dendrites and intricately tile the larval body. As Drosophila larvae progress through developmental stages, the dendritic arbors of Class IV neurons undergo notable transformations. As Drosophila larvae develop, their Class IV dendritic arbors grow. In the initial 24 h after egg laying (AEL), the dendrites are smaller than segments. During the subsequent 24 h of the first instar larval stage, dendritic arbors outpace segment growth, achieving tiling. After 48 h, arbors and segments grow concurrently. Epidermal cells near Class IV dendrites expand in proportion to segment growth. This observation suggested that Class IV cells might grow via branch dilation—uniformly elongating branches, akin to Class I cells [1,2]. To understand whether the class IV complex arbor structure is formed by dilation or simply from growing tips, we developed this protocol to introduce a systematic approach for quantitatively assessing the growth dynamics of internal branches.
KW  - Class IV neurons
KW  - Branching morphogenesis
KW  - Neuronal development
KW  - Dendrite
KW  - Drosophila larvae
KW  - Tip growth
KW  - Confocal microscopy
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False