TY  - JOUR
TI  - Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle
AU  - Zhang, Raymond Jiahong
AU  - Vermot, Julien 
AU  - Gherardi, Riccardo 
AU  - Fukui, Hajime 
AU  - Chow, Renee Wei-Yan
VL  - 14
IS  - 10
PY  - 2024
DA  - 2024/05/20
SP  - e4989
C1  - Bio-protocol 2024;14:e4989
DO  - 10.21769/BioProtoc.4989
UR  - https://doi.org/10.21769/BioProtoc.4989
AB  - Calcium signalling in the endocardium is critical for heart valve development. Calcium ion pulses in the endocardium are generated in response to mechanical forces due to blood flow and can be visualised in the beating zebrafish heart using a genetically encoded calcium indicator such as GCaMP7a. Analysing these pulses is challenging because of the rapid movement of the heart during heartbeat. This protocol outlines an imaging analysis method used to phase-match the cardiac cycle in single z-slice movies of the beating heart, allowing easy measurement of the calcium signal.
KW  - Software development
KW  - Cardiac cycle
KW  - Heart valve development
KW  - Phase matching
KW  - Image analysis
KW  - Zebrafish embryos
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False