TY  - JOUR
TI  - Apolipoprotein B Secretion Assay from Primary Hepatocytes
AU  - Wang, Yawei 
AU  - Li, Xin 
AU  - Huang, Runze 
AU  - Chen, Xiao- Wei
AU  - Wang, Xiao 
VL  - 14
IS  - 9
PY  - 2024
DA  - 2024/05/05
SP  - e4982
C1  - Bio-protocol 2024;14:e4982
DO  - 10.21769/BioProtoc.4982
UR  - https://doi.org/10.21769/BioProtoc.4982
AB  - Apolipoprotein B (APOB) is the primary structural protein of atherogenic lipoproteins, which drive atherogenesis and thereby lead to deadly cardiovascular diseases (CVDs). Plasma levels of APOB-containing lipoproteins are tightly modulated by LDL receptor–mediated endocytic trafficking and cargo receptor–initiated exocytic route; the latter is much less well understood. This protocol aims to present an uncomplicated yet effective method for detecting APOB/lipoprotein secretion. We perform primary mouse hepatocyte isolation and culture coupled with well-established techniques such as immunoblotting for highly sensitive, specific, and semi-quantitative analysis of the lipoprotein secretion process. Its inherent simplicity facilitates ease of operation, rendering it a valuable tool widely utilized to explore the intricate landscape of cellular lipid metabolism and unravel the mechanistic complexities underlying lipoprotein-related diseases.
KW  - Cardiovascular diseases
KW  - Hyperlipidemia
KW  - Lipoprotein secretion
KW  - Primary hepatocytes
KW  - Western blot
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False