TY  - JOUR
TI  - Ten-fold Robust Expansion Microscopy
AU  - Damstra, Hugo G. J.
AU  - Mohar, Boaz 
AU  - Eddison, Mark 
AU  - Akhmanova, Anna 
AU  - Kapitein, Lukas C
AU  - Tillberg, Paul W.
VL  - 13
IS  - 12
PY  - 2023
DA  - 2023/06/20
SP  - e4698
C1  - Bio-protocol 2023;13:e4698
DO  - 10.21769/BioProtoc.4698
UR  - https://doi.org/10.21769/BioProtoc.4698
AB  - Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy that can be applied in both tissues and cells. In ExM, samples are embedded in a swellable polymer gel to physically expand the sample and isotropically increase resolution in x, y, and z. By systematic exploration of the ExM recipe space, we developed a novel ExM method termed Ten-fold Robust Expansion Microscopy (TREx) that, as the original ExM method, requires no specialized equipment or procedures. TREx enables ten-fold expansion of both thick mouse brain tissue sections and cultured human cells, can be handled easily, and enables high-resolution subcellular imaging with a single expansion step. Furthermore, TREx can provide ultrastructural context to subcellular protein localization by combining antibody-stained samples with off-the-shelf small molecule stains for both total protein and membranes.
KW  - Expansion microscopy
KW  - Super resolution
KW  - Light microscopy
KW  - Antibody labeling
KW  - Ultrastructure
KW  - Protein stain
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False