TY  - JOUR
TI  - Visualization of Lipid Droplets in the Alveolar Macrophage Cell Line MH-S with Live-cell Imaging by 3D Holotomographic Microscopy (Nanolive)
AU  - Pérez-Montero, Andrea 
AU  - Zaragoza, Oscar 
AU  - Luque, Alfonso 
AU  - Hortelano, Sonsoles 
AU  - Acebo, Paloma 
VL  - 13
IS  - 5
PY  - 2023
DA  - 2023/03/05
SP  - e4629
C1  - Bio-protocol 2023;13:e4629
DO  - 10.21769/BioProtoc.4629
UR  - https://doi.org/10.21769/BioProtoc.4629
AB  - Lipid droplets (LD), triglycerides and sterol esters among them, are well known for their capacity as lipid storage organelles. Recently, they have emerged as critical cytoplasmic structures involved in numerous biological functions. LD storage is generated de novo by the cell and provides an energy reserve, lipid precursors, and cell protection. Moreover, LD accumulation can be observed in some pathologies as obesity, atherosclerosis, or lung diseases. Fluorescence imaging techniques are the most widely used techniques to visualize cellular compartments in live cells, including LD. Nevertheless, presence of fluorophores can damage subcellular components and induce cytotoxicity, or even alter the dynamics of the organelles. As an alternative to fluorescence microscopy, label-free techniques such as stimulated Raman scattering and coherent anti-stokes Raman scattering microscopy offer a solution to avoid the undesirable effects caused by dyes and fluorescent proteins, but are expensive and complex. Here, we describe a label-free method using live-cell imaging by 3D holotomographic microscopy (Nanolive) to visualize LD accumulation in the MH-S alveolar macrophage cell line after treatment with oleic acid, a monounsaturated fatty acid that promotes lipid accumulation.
KW  - Lipid droplet
KW  - Alveolar macrophages
KW  - Oleic acid
KW  - Metabolism
KW  - 3D holotomographic microscopy
KW  - Lung pathologies
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False