TY  - JOUR
TI  - 14C-paraquat Efflux Assay in Arabidopsis Mesophyll Protoplasts
AU  - Xia, Jin- Qiu
AU  - Liu, Qian- Qian
AU  - Xiang, Cheng- Bin
VL  - 12
IS  - 18
PY  - 2022
DA  - 2022/09/20
SP  - e4512
C1  - Bio-protocol 2022;12:e4512
DO  - 10.21769/BioProtoc.4512
UR  - https://doi.org/10.21769/BioProtoc.4512
AB  - Weeds compete with crops for growth resources, causing tremendous yield losses. Paraquat is one of the three most common non-selective herbicides. To study the mechanisms of paraquat resistance, we need to trace the movement of paraquat in plants and within the cell. 14C is a radioactive carbon isotope widely used to trace substances of interest in various biological studies, especially in transport analyses. Here, we describe a detailed protocol using 14C-paraquat to demonstrate paraquat efflux in Arabidopsis protoplasts.
KW  - 14C-paraquat
KW  - Isotope
KW  - Protoplasts
KW  - Efflux transport
KW  - Arabidopsis
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False