TY  - JOUR
TI  - Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex
AU  - Namba, Takashi 
AU  - Haffner, Christiane 
AU  - Huttner, Wieland B
VL  - 11
IS  - 10
PY  - 2021
DA  - 2021/05/20
SP  - e4031
C1  - Bio-protocol 2021;11:e4031
DO  - 10.21769/BioProtoc.4031
UR  - https://doi.org/10.21769/BioProtoc.4031
AB  - The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note: This protocol requires basic knowledge of brain anatomy), we describe three ex vivo neocortical slice/tissue culture methods: (i) organotypic slice culture (mouse, ferret, human); (ii) hemisphere rotation culture (mouse, ferret); and (iii) free-floating tissue culture (mouse, ferret, human). Each of these three culture methods offers distinct features with regard to the analyses to be performed and can be combined with genetic manipulation by electroporation and treatment with specific inhibitors. These three culture methods are therefore powerful techniques to examine the function of genes involved in neocortical development.
KW  - Human
KW  - Mouse
KW  - Ferret
KW  - Neocortex
KW  - Development
KW  - Evolution
KW  - Neural stem/Progenitor cells
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False