TY  - JOUR
TI  - Generation of Mouse Primary Hypothalamic Neuronal Cultures for Circadian Bioluminescence Assays
AU  - Schmidt, Cosima Xenia
AU  - Tsang, Anthony H.
AU  - Oster, Henrik  
VL  - 11
IS  - 5
PY  - 2021
DA  - 2021/03/05
SP  - e3944
C1  - Bio-protocol 2021;11:e3944
DO  - 10.21769/BioProtoc.3944
UR  - https://doi.org/10.21769/BioProtoc.3944
AB  - An endogenous circadian clock system enables organisms to adapt to time-of-day dependent environmental changes. In consequence, most physiological processes exhibit daily rhythms of, e.g., energy metabolism, immune function, sleep, or hormone production. Hypothalamic circadian clocks have been identified to play a particular role in coordinating many of these processes. Primary neuronal cultures are widely used as a physiologically relevant model to study molecular events within neurons. However, as circadian rhythms include dynamic molecular changes over longer timescales that vary between individual cells, longitudinal measurement methods are essential to investigate the regulation of circadian clocks of hypothalamic neurons. Here we provide a protocol for generating primary hypothalamic neuronal cultures expressing a circadian luciferase reporter. Such reporter cells can be used to longitudinally monitor cellular circadian rhythms at high temporal resolution by performing bioluminescence measurements.
KW  - Primary hypothalamic neurons
KW  - Circadian clocks
KW  - Luciferase reporter
KW  - Bmal1
KW  - Circadian rhythms
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False