TY  - JOUR
TI  - Induction of Epithelial-mesenchymal Transition in MDCK II Cells
AU  - Pastuła, Agnieszka  
AU  - Lundmark, Richard 
VL  - 11
IS  - 3
PY  - 2021
DA  - 2021/02/05
SP  - e3903
C1  - Bio-protocol 2021;11:e3903
DO  - 10.21769/BioProtoc.3903
UR  - https://doi.org/10.21769/BioProtoc.3903
AB  - Epithelial-mesenchymal transition (EMT) is a reversible process of epithelial cell transdifferentiation into a mesenchymal cell, that enables initiation of cell migration. EMT plays an important role in embryonic development, tissue repair and cancer metastasis. Better understanding of cellular and molecular events during EMT will not only provide novel insights on how mammalian organism develops and how epithelial tissues regenerate, but also can identify novel therapeutic targets for cancer therapy. Here we aim to provide a detailed protocol on how to induce EMT in Madin-Darby Canine Kidney (MDCK) II epithelial cell line and perform immunofluorescent staining on EMT-induced cells.
KW  - Epithelial-mesenchymal transition
KW  - MDCK cells
KW  - Hepatocyte growth factor
KW  - Epithelial plasticity
KW  - EMT
KW  - Mesenchymal cell differentiation from epithelial cell
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False