TY  - JOUR
TI  - Characterization of Hippocampal Adult-borne Granule Cells in a Transient Cerebral Ischemia Model
AU  - Ceanga, Mihai 
AU  - Guenther, Madlen 
AU  - Ingrisch, Ina 
AU  - Kunze, Albrecht 
VL  - 11
IS  - 2
PY  - 2021
DA  - 2021/01/20
SP  - e3890
C1  - Bio-protocol 2021;11:e3890
DO  - 10.21769/BioProtoc.3890
UR  - https://doi.org/10.21769/BioProtoc.3890
AB  - Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric long-term sequelae of stroke. Reliable animal models of stroke are paramount to understanding their biomechanisms and to advancing therapeutic strategies. We present a detailed protocol of a transient cerebral ischemia model which does not cause direct ischemic damage in the hippocampus, allowing investigations into the pathophysiology of long-term neurocognitive deficits of stroke. Furthermore, we describe a protocol for obtaining acute hippocampal slices for the purpose of electrophysiological and morphological characterization of adult-borne granule cells. Particularities relating to performing electrophysiological recordings from small cells, such as immature adult-borne granule cells, are also discussed. The present protocol may be complemented by multi-modal investigations (behavioral, morpho-structural, biochemical), to hopefully facilitate research and advances into the long-term sequelae of stroke and the discovery of new therapeutic opportunities.
KW  - Stroke
KW  - MCAO
KW  - Adult-borne granule cells
KW  - Acute hippocampal slices
KW  - Whole cell patch clamp
JF  - Bio-protocol
SN  - 2331-8325
PB  - Bio-protocol LLC.
BIO101  - False